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CJ236 Electrocompetent Cells

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CJ236 Electrocompetent Cells

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产品名称:

CJ236 Electrocompetent Cells

货号
60701-2
规格
24 rxns (DUOs)
存储温度
-80º C
没有此类产品
产品描述

CJ236 Electrocompetent Cells

 

产品说明书

 

High efficiency cells to create uracil-containing DNA for site-directed mutagenesis

  • Sole source of highly efficient Electrocompetent cells (1 × 109 cfu/µg)
  • ung- and dut- mutations to generate DNA with some uracil residues
  • Ideal genotype and efficiency for Kunkel directed mutagenesis method1.

1 Kunkel T.A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection.  Proc. Natl. Acad. Sci.52:488-492.

Lucigen is the sole provider of high-efficiency electrocompetent CJ236 cells. This cell line was created by adding the pCJ105 plasmid to BW313 cells2, and is ideally suited to the Kunkel directed mutagenesis method. This method relies on the cells producing plasmid DNA containing some uracil residues. The dut- and ung- mutations prevent the cells from removing uracil from DNA caused by misincorporation or dCTP deamination. Note that these cells contain an F’ which confers resistance to Chloramphenicol at low levels.

As Electrocompetent cells, the transformation efficiency is 1 × 109 cfu/µg.

Functional genotype:
[F’ Tra+ Pil+ (CamR)] ung-1 relA1 dut-1 thi-1 spoT1 mcrA

2 Joyce, C.M., N. Grindley 1984. Method for determining whether a gene of Escherichia coli is essential: application to the polA gene. J.Bacteriol. 158: 636-643

  • Ordering

  • Specifications

  • Manuals

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Contact your local distributor for pricing
Product Description Size Cat. No.
CJ236 Electrocompetent Cells   12 rxns 60701-1
    24 rxns 60701-2
ORDER INFORMATION
Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
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