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SS320 (MC1061 F′) Electrocompetent Cells

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SS320 (MC1061 F′) Electrocompetent Cells

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产品名称:

SS320 (MC1061 F′) Electrocompetent Cells

货号
60512-2
规格
24 rxns (DUOs)
存储温度
-80º C
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产品描述

Phage Display Competent Cells

 

产品说明书

 

  • Perfect for antibody phage display or peptide phage display library creation
  • The highest efficiency TG1 competent cells available: ≥4 × 1010 cfu/µg.
  • Your only source for electrocompetent SS320 and ER2738 cells
  • Amber suppressor and non-amber suppressor lines for maximum flexibility
  • High efficiency MC1061 F- cells for cloning or control reactions
  • Create larger libraries; speed discovery
  • Bulk custom dispensing available with all of our phage display cells
 

MC1061 F- Electrocompetent Cells. A non-amber suppressor strain, identical to SS320 except that it lacks the F′episome. Highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for general cloning or Phage Display. Note that as an F- strain, it cannot be used for re-infection by filamentous phage.  This strain not included in the sample kit.

  • TG1 Electrocompetent Cells. An amber suppressor strain (supE) prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells are used for phage display and protein expression.

  • SS320 Electrocompetent Cells. A non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells have the highest available transformation efficiency of any strain used for phage display.
  • ER2738 Electrocompetent Cells. An amber suppressor strain (glnV) prepared as highly efficient (≥2 × 1010 cfu/µg) electrocompetent cells for phage display library screening. This strain is recommended for use with New England Biolab's Ph.D.™ Phage Display Kits.
Figure 1. Transformation efficiency of Lucigen's electrocompetent bacterial cells for phage display compared to competitor's specification.

Table 1. Competent Cells for Phage Display libraries


Cell Lines

Transformation Efficiency
(cfu/µg pUC DNA)

Cloning Methylated DNA

BAC, Cosmid Cloning

Blue/White Screening

TG1 Electrocompetent

>4 × 1010

YES

NO

YES
IPTG induction required

SS320 (MC1061 F') Electrocompetent

>4× 1010

YES

NO

YES
IPTG induction required

MC1061 F- Electrocompetent

>4× 1010

YES

NO

YES
IPTG induction required

ER2738 Electrocompetent

>2 × 1010

YES

NO

YES
IPTG induction required

Custom Competent Cells

Bulk Lucigen cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing!See Custom Competent Cells for detailed information.

 
  • Ordering

  • Specifications

  • Manuals

  • Resources

Contact your local distributor for pricing
Product Description Size Cat. No.
Phage Display ElectroCombo Pack (four transformations of each strain [TG1, SS320, ER2738])   12 rxns 60500-0
ER2738 Electrocompetent Cells   12 rxns (DUOs) 60522-1
    24 rxns (DUOs) 60522-2
SS320 (MC1061 F') Electrocompetent Cells   12 rxns (DUOs) 60512-1
    24 rxns (DUOs) 60512-2
TG1 Electrocompetent Cells   12 rxns (DUOs) 60502-1
    24 rxns (DUOs) 60502-2
MC1061 F- Electrocompetent Cells   24 rxns (DUOs) 60514-2
    12 rxns (DUOs) 60514-1
ORDER INFORMATION

Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all Lucigen Competent Cells. 

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