QQ:1443274331

010-88891086,80794780

服务电话:

搜索

 北京市昌平区北清路生命科学园北清创意园2-1-102;  Tel:010-88891086,80794780   Fax:010-80794780 
咨询订购QQ: 1443274331   技术支持QQ: 2074750152   邮件订购:bjzblg@126.com      
www.epibio.com.cn  www.epicentre.cn  www.immunoreagents.cn  www.lucigen.com.cn
版权所有 @北京中北林格科技发展有限公司   
京ICP备:13002510号       网站建设:中企动力 北京

进口试剂

>
>
>
>
Expresso® Solubility and Expression Screening System + SelecTEV™ Protease and Accura® High-Fidelity Polymerase

进口试剂

Expresso® Solubility and Expression Screening System + SelecTEV™ Protease and Accura® High-Fidelity Polymerase

浏览量
产品名称:

Expresso® Solubility and Expression Screening System + SelecTEV™ Protease and Accura® High-Fidelity Polymerase

货号
49066-1
规格
24 rxns
存储温度
-20º C
没有此类产品
产品描述

Expresso® Solubility and Expression Screening System

 

产品说明书

 

Multiplex your protein solubility screen

这是一个方便的筛选盒子,含有七个融合性标签可以增加蛋白表达和可溶性。

  • 使用一个扩增子获得您所构建的全部产物
  • 通过TEV切割融合标签进行快速,同步的蛋白筛选
  • 从PCR到蛋白获得不到4天时间。

 

  • Overview
  • Simplified workflow
  • Results
  • Fusion tags included
  • Protein cleavage with SelecTEV™ Protease
  • FAQ's

概述

The Expresso® 可溶性表达系统是一个完整的系统可以完成PCR扩增,快速克隆以及表达您所感兴趣的蛋白,通过一个独特的标签切割系统来实现。Lucigen已经鉴定了7个独特有效的可溶性标签,并且将他们与表达技术结合创立了一套克隆系统用于快速同时评价你的蛋白带有7个可溶标签(加上一个6组氨酸标签的对照载体)。

pSOL Vector

Figure 1. Each pSOL vector contains everything you need to seamlessly clone in your target gene without any additional amino acids added.  Fusion tags are cleavable via a TEV protease site. 


Simplified Workflow

Expresso Screening Workflow

Lucigen的表达技术使这项工作比以往更简单快速,可以快速筛选多融合标签,提高了蛋白的表达和可溶性。使用一个扩增子同时产生8个不同克隆(7个融合标签的和一个带组氨酸标签的对照),接下来筛选表达的可溶性蛋白

 

 


结果


每个蛋白对于不同的融合标签反应不同。使用可溶性表达筛选系统,你可以快速,容易的大量筛选,筛选到对于你的蛋白最适的融合标签。

GH1
Figure 2. GH1 (Human Growth Hormone) was evaluated for E. coli expression and solubility when expressed as a fusion. Fusion tags SlyD, Tsf, SUMO, Bla, and MBP demonstrated enhanced solubility (Lane S) compared to the His-control protein. T: Total Lysate, S: Cleared Soluble Fraction, I: Insoluble Pellet

Fusion Tags Included

The Expresso Solubility and Expression Screening System includes 7 vectors that each contain a unique fusion tag and one His control vector. The fusion tags vary in sequence, size, and derivative. Below is a table summarizing the characteristics of each fusion tag:

#

Fusion Tag

AA Length

kDa*

pI

Description

1

6xHis-AFV

113

13.5

5.0

Hypothetical protein from Acidianus filamentous virus 1

2

6xHis-SlyD

210

22.7

5.2

FKBP-type peptidyl-prolyl cis-trans isomerase

3

6xHis-Tsf

297

32.2

5.7

E. coli elongation factor

4

6xHis-SUMO

115

13.3

5.2

Small Ubiquitin-like Modifier

5

6xHis-Bla

381

41.3

4.4

Beta-lactamase

6

6xHis-MBP

382

42.1

5.5

Maltose-Binding Protein

7

6xHis-GST

233

27.4

6.6

Glutathione S-transferase

8

6xHis Control

14

1.8

7.0

Affinity tag


Cleavage with SelecTEV® Protease

All fusion tagged proteins produced with the Expresso Solubility and Expression Screening System are fully cleavable with SelecTEV protease from Lucigen.  For more information, visit the SelecTEV protease product page

SelecTEV Protease
Figure 3. Coomassie-stained SDS-PAGE gel of TEV cleavage reaction. 10 U of SelecTEV Protease was incubated with 30 µg of fusion protein substrate at 30°C for 1 hour in a 100 µL reaction. Marker (lane M), SelecTEV Protease (Lane 1), Uncleaved fusion substrate (Lane 2), Cleaved substrate and SelecTEV Protease. (Lane 3)
  • Ordering

  • Manuals

  • Resources

包含的融合蛋白
可溶性表达筛选系统包括 7个载体,每一个载体含有一个独特的可溶标签和一个组氨酸对照载体。融合标签在序列,大小和来源上都不同。下面的表格总结了每种融合标签的特点。

Contact your local distributor for pricing

Product Description Size Cat. No.
Expresso® Solubility and Expression Screening System   24 rxns (3 rxns for each vector) 49060-1
Expresso® Solubility and Expression Screening System + Accura® High-Fidelity Polymerase   24 rxns + 25 units Accura 49064-1
Expresso® Solubility and Expression Screening System + SelecTEV™ Protease   24 rxns + 1,000 Units SelecTEV Protease 49062-1
Expresso® Solubility and Expression Screening System + SelecTEV™ Protease + Accura® High-Fidelity Polymerase   24 rxns + 1,000 Units SelecTEV + 25 units Accura 49066-1
ORDER INFORMATION

The Expresso Solubility and Expression Screening System contains pre-processed pSol™ vectors with 7 different fusion tags and one HIS-tagged control vector (3 reactions of each vector), positive control insert, sequencing primers, E. cloni® 10G Chemically Competent Cells (SOLOs), and 20% Rhamnose solution and 15% Glucose solution autoinduction reagents. SelecTEV™ Protease and Accura® High-Fidelity Polymerase are available for purchase separately.

For research use only. Complete protocols are available online at www.lucigen.com/manuals.

未找到相应参数组,请于后台属性模板中添加
暂未实现,敬请期待
暂未实现,敬请期待
下一篇