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进口试剂

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CopyRight® v2.0 Fosmid Cloning Kit

进口试剂

CopyRight® v2.0 Fosmid Cloning Kit

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产品名称:

CopyRight® v2.0 Fosmid Cloning Kit

货号
42027-1
规格
5 rxns
存储温度
-20º & -80º C
没有此类产品
产品描述

CopyRight® v2.0 Fosmid Cloning Kits (pSMART® FOS)

 

产品说明书

 

  • 有效克隆大小在35-45kb的片段
  • 少量DNA得到更多的重组子
  • 插入稳定性高
  • 诱导拷贝数

改进 Fosmid克隆

Lucigen’s CopyRight® v2.0 Fosmid克隆试剂盒,含有 pSMART® FOS载体和Replicator™ FOS菌株,含有最先进的fosmid克隆技术。The CopyRight v2.0 Fosmid盒子可以从有效的DNA产生大量的重组子(如图1), 载体整合了CopyRight转录终止子确保插入的稳定性,也保证了完整的fosmid文库的构建。基本消除了假阳性和空载体的情况(如图2)。Fos载体的可控遗传元件pSMART和fos细胞的复制子可以保证载体扩增的拷贝数,获得大量质粒(如图4)

图1.利用有限的DNA量获得更高的重组子。Lucigen’s CopyRight Fosmid kit 与 Epicentre’s CopyControl kit比较图。

Figure 2. 使用lucigen pSMART FOS载体(左图)与epicentre CopyControl™ pCC1FOS™载体(右图)比较,lucigen载体无空白插入。

                                    Figure 3. CopyRight v2.0 pSMART FOS 载体图谱

 

Figure 4. 使用the CopyRight v2.0 Fosmid盒子产生的fosmid克隆拷贝数扩增与epicentre比较,复制子细胞更稳定,更好

易于成功
有效的获得克隆或创建文库- CopyRight v2.0克隆盒子消除了繁琐的载体制备和感受态细胞制备,节省了时间。盒子包含了优化的试剂,连接载体,优化的细胞,详细的说明书和疑难解答知道。

  • Ordering

  • Manuals

  • Resources

每个CopyRight v2.0 Fosmid克隆试剂盒包含,预先切割,去磷酸化的平末端pSMART FOS载体,CloneSmartDNA连接酶,10X连接buffer(含有ATP),DNA终止子,末端修复酶混液和末端修复buffer,复制菌株fos 株甘油管,20%麦芽糖溶液,1M 硫酸镁,SM buffer,阿拉伯糖诱导溶液,测序引物和完整的操作手册。20次的盒子是由两个10次盒子组成。

Contact your local distributor for pricing

Product Description Size Cat. No.
BAC-Optimized Replicator v2.0 Electrocompetent Cells   12 rxns (DUOs) 60210-1
    24 rxns (DUOs) 60210-2
CopyRight v2.0 BAC Cloning Kit, pSMART BAC BamHI w/BAC-Optimized Replicator v2.0 Electrocompetent Cells   10 rxns 42030-1
    20 rxns 42030-2
CopyRight v2.0 BAC Cloning Kit, pSMART BAC HindIII w/BAC-Optimized Replicator v2.0 Electrocompetent Cells   10 rxns 42032-1
    20 rxns 42032-2
CopyRight v2.0 pEZ BAC Blunt Cloning Kit w/Electrocompetent Cells   10 rxns 42009-1
    20 rxns 42009-2
CopyRight v2.0 pEZ BAC Blunt Cloning Kit without cells   10 rxns 42016-1
ORDER INFORMATION

Each CopyRight® v2.0 BAC Cloning Kit contains: pre-cut vector (pSMART BAC with a choice of BamHI or HindIII sites), CopyRight DNA Ligase, CopyRight 5X Ligation Buffer, Positive Control Insert DNA, Sequencing Primers, BAC-Optimized Replicator v2.0 Electrocompetent Cells in DUO packaging (2 transformations per tube), Positive Control Insert DNA, Recovery Medium, Arabinose Induction Solution, and complete protocols. Each CopyRight pEZ BAC Cloning Kit contains: pre-cut CopyRight vector (pEZ BAC Blunt), CloneSmart DNA Ligase, CloneDirect™ 10X Ligation Buffer (includes ATP), Positive Control Insert DNA, Replicator Induction Solution, Sequencing Primers, and complete protocols. Kits with cells also include BAC-Optimized Replicator v2.0 Electrocompetent Cells (>1 × 1010 cfu/µg DNA) packaged in two transformations per tube (DUOs), Positive Control Plasmid, and Recovery Medium.

Please note that the following vectors have been discontinued: pSMART BAC EcoRI (catalog numbers 42031-1, 42031-2) and pEZ BAC BamHI (catalog numbers 42007-1, 42007-2 and 42012-1).”

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