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进口试剂

进口试剂

SelecTEV™ Protease

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产品名称:

SelecTEV™ Protease

货号
30810-1
规格
1,000 U
存储温度
-20º C
没有此类产品
产品描述

SelecTEV™ Protease

 

产品说明书

 

  • High specific activity and specificity
  • Engineered for stability
  • 6-Histidine tag for easy removal
 

SelecTEV™ Protease is a 27 kD improved form of Tobacco Etch Virus (TEV) protease that has been engineered to be more stable, active, and specific than the native protease. SelecTEV™ Protease is a cysteine protease that recognizes the TEV recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. This seven-amino-acid sequence is rarely found in proteins, making SelecTEV™ Protease an ideal choice for tag removal of fusion proteins. The optimal temperature for cleavage is 30°C, however the enzyme is active over a wide temperature range (4 – 30°C) and pH (6.0 – 8.5) to accommodate your specific protein. Following digestion, SelecTEV™ Protease is easily removed from the cleavage reaction by affinity chromatography using the polyhistidine tag at the N-terminus of the protease. SelecTEV™ Protease is purified from E. coli by affinity chromatography using the polyhistidine tag and is 90% pure when visualized on an SDS-PAGE gel.

SelecTEV Protease can be used with the Expresso Solubility and Expression Screening System vectors, which contain a TEV cleavage recognition site.

SelecTEV Protease

Coomassie-stained SDS-PAGE gel of TEV cleavage reaction. 10 U of SelecTEV Protease was incubated with 30 µg of fusion protein substrate at 30°C for 1 hour in a 100 µL reaction. Marker (lane M), SelecTEV Protease (Lane 1), Uncleaved fusion substrate (Lane 2), Cleaved substrate and SelecTEV Protease. (Lane 3)

  • Ordering

  • Specifications

  • Manuals

  • Resources

Contact your local distributor for pricing
Product Description Size Cat. No.
SelecTEV™ Protease   1,000 U 30810-1
    5,000 U 30810-2
ORDER INFORMATION

30810-1: SelecTEV Protease (1 tube), 100 mM DTT (1 tube), 20X SelecTEV Buffer (1 tube)

30810-2: SelecTEV Protease (5 tubes), 100 mM DTT (5 tubes), 20X SelecTEV Buffer (5 tubes)

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