QQ：1443274331
010-88891086，80794780
服务电话：
北京市昌平区北清路生命科学园北清创意园2-1-102; Tel：010-88891086，80794780 Fax：010-80794780
咨询订购QQ: 1443274331 技术支持QQ: 2074750152 邮件订购：bjzblg@126.com
www.epibio.com.cn www.epicentre.cn www.immunoreagents.cn www.lucigen.com.cn
版权所有 @北京中北林格科技发展有限公司 京ICP备：13002510号 网站建设：中企动力 北京
进口试剂
进口试剂
NxGen® T7 RNA Polymerase
NxGen® T7 RNA Polymerase
-
Reliable transcription.
T7 RNA Polymerase catalyzes the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognizes the T7 promoter and terminator sequences with high specificity.
 |
Specifications
Unit Definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C.
Source: Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.
| Unit Concentration | 50,000 U/mL |
| Purity (SDS-PAGE) | >99% |
| SS Exonuclease | 500 U <0.1% released |
| DS Exonuclease | 500 U <0.1% released |
| Endonuclease | 500 U <10% converted |
| RNAse Contamination | 500 U = none detected |
| E. coli 16S rDNA Contamination | 500 U <10 copies |
| Storage | -20°C |
-
Ordering
-
Manuals
-
Resources
Supplied at 50,000 U/mL in 50 mM Tris-HCl, 100 mM NaCl, 1 mM dithiothreitol, 1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.9 @ 25°C. Also provided is 10X T7 RNA Polymerase Buffer which in 1X form is composed of 40 mM Tris-HCl, 6 mM MgCl2, 10 mM Dithiothreitol, 2 mM Spermidine, pH 7.9 @ 25°C. 125,000 U is supplied as 5 × 25,000 U.