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NxGen® T7 RNA Polymerase

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NxGen® T7 RNA Polymerase

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产品名称:

NxGen® T7 RNA Polymerase

货号
30223-1
规格
25,000 U
存储温度
-20º C
没有此类产品
产品描述

NxGen® T7 RNA Polymerase

 

产品说明书

 

  • Reliable transcription.

 

NxGen Enzymes IconT7 RNA Polymerase catalyzes the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognizes the T7 promoter and terminator sequences with high specificity.

T7 RNA Polymerase

Specifications

Unit Definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C.

Source: Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.

Unit Concentration 50,000 U/mL
Purity (SDS-PAGE) >99%
SS Exonuclease 500 U <0.1% released
DS Exonuclease 500 U <0.1% released
Endonuclease 500 U <10% converted
RNAse Contamination 500 U = none detected
E. coli 16S rDNA Contamination 500 U <10 copies
Storage -20°C
  • Ordering

  • Manuals

  • Resources

Contact your local distributor for pricing
Product Description Size Cat. No.
NxGen® T7 RNA Polymerase   25,000 U 30223-1
    125,000 U 30223-2
Pyrophosphatase Inorganic, Thermostable   250 Units 30074-1
    (5 x 250 U) 30074-2
NxGen® RNAse Inhibitor   10,000 U 30281-1
    50,000 U 30281-2
ORDER INFORMATION
Supplied at 50,000 U/mL in 50 mM Tris-HCl, 100 mM NaCl, 1 mM dithiothreitol, 1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.9 @ 25°C. Also provided is 10X T7 RNA Polymerase Buffer which in 1X form is composed of 40 mM Tris-HCl, 6 mM MgCl2, 10 mM Dithiothreitol, 2 mM Spermidine, pH 7.9 @ 25°C. 125,000 U is supplied as 5 × 25,000 U.
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