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进口试剂
T4 DNA Polymerase, Exonuclease Minus
T4 DNA Polymerase, Exonuclease Minus
- Only source for recombinant T4 DNA Polymerase, Exonuclease Minus.
- Potential applications include enhancing DNA sequencing reads.
T4 DNA Polymerase, Exonuclease Minus is a DNA-dependent DNA polymerase which catalyzes the 5’→3’ synthesis of DNA and requires template and primer. Additionally, the enzyme lacks 3’→5’ exonuclease activity.
Source:
Purified from a strain of E. coli that carries a T4 DNA Polymerase, Exonuclease Minus expression plasmid.
Applications:
- DNA Sequencing reaction.(1)
Heat inactivation: 70°C for 15 minutes.
Storage:
Enzyme and buffer should be stored at -20°C. Enzyme is stable for 12 months if handled properly.
Additional Information:
- May increase the DNA sequencing read-lengths.
References
- Nancy G. Nossal (1998) Genetics 148: 1535-1538.
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Ordering
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Manuals
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Resources
Recombinant T4 DNA Polymerase, Exonuclease Minus is supplied at a concentration of 3,000U/ml in a storage buffer of 50% glycerol, 100 mM KPO4 (pH 6.5) and 1 mM DTT. Also supplied is 10X DNA Polymerase Buffer A composed of 100 mM Tris-HCl (pH7.9), 100 mM MgCl2, 500 mM NaCl and 10 mM DTT.