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T4 DNA Polymerase, Exonuclease Minus

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T4 DNA Polymerase, Exonuclease Minus

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产品名称:

T4 DNA Polymerase, Exonuclease Minus

货号
30085-3
规格
1,500 U
存储温度
-20º C
没有此类产品
产品描述

T4 DNA Polymerase, Exonuclease Minus

 

产品说明书

 

  • Only source for recombinant T4 DNA Polymerase, Exonuclease Minus.
  • Potential applications include enhancing DNA sequencing reads.
 

T4 DNA Polymerase, Exonuclease Minus is a DNA-dependent DNA polymerase which catalyzes the 5’→3’ synthesis of DNA and requires template and primer.  Additionally, the enzyme lacks 3’→5’ exonuclease activity.

Source:
Purified from a strain of E. coli that carries a T4 DNA Polymerase, Exonuclease Minus expression plasmid.

Applications:

  • DNA Sequencing reaction.(1)

Heat inactivation: 70°C for 15 minutes.

Storage:
Enzyme and buffer should be stored at -20°C. Enzyme is stable for 12 months if handled properly.

Additional Information:

  • May increase the DNA sequencing read-lengths.

References

  1. Nancy G. Nossal (1998) Genetics 148: 1535-1538.
  • Ordering

  • Manuals

  • Resources

Contact your local distributor for pricing
Product Description Size Cat. No.
T4 DNA Polymerase, Exonuclease Minus   300 U 30085-1
    1500 U 30085-3
ORDER INFORMATION
Recombinant T4 DNA Polymerase, Exonuclease Minus is supplied at a concentration of 3,000U/ml in a storage buffer of 50% glycerol, 100 mM KPO4 (pH 6.5) and 1 mM DTT. Also supplied is 10X DNA Polymerase Buffer A composed of 100 mM Tris-HCl (pH7.9), 100 mM MgCl2, 500 mM NaCl and 10 mM DTT.
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