QQ:1443274331

010-88891086,80794780

服务电话:

搜索

 北京市昌平区北清路生命科学园北清创意园2-1-102;  Tel:010-88891086,80794780   Fax:010-80794780 
咨询订购QQ: 1443274331   技术支持QQ: 2074750152   邮件订购:bjzblg@126.com      
www.epibio.com.cn  www.epicentre.cn  www.immunoreagents.cn  www.lucigen.com.cn
版权所有 @北京中北林格科技发展有限公司   
京ICP备:13002510号       网站建设:中企动力 北京

进口试剂

进口试剂

T4 Polynucleotide Kinase

浏览量
产品名称:

T4 Polynucleotide Kinase

货号
30061-3
规格
5,000 U
存储温度
-20º C
没有此类产品
产品描述

T4 Polynucleotide Kinase

 

产品说明书

 

  • Recombinant T4 Polynucleotide Kinase (T4 PNK).
  • Applications include end-labeling and insert preparation for cloning.
 

T4 Polynucleotide Kinase catalyzes the transfer and exchange of gamma-phosphate of ATP to the 5´-hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3´-monophosphates.

Source:
Purified from a strain of E. coli that carries a T4 Polynucleotide Kinase expression plasmid.

Applications:

  • End-labeling DNA for probes and DNA sequencing (1)
  • Addition of 5´-phosphates to oligonucleotides to allow subsequent ligation (2)

Heat inactivation: 70°C for 15 minutes.

Storage:
Enzyme and buffer should be stored at -20°C. Enzyme is stable for 12 months if handled properly.

Additional Information:

  • Ammonium ions are potent inhibitors of T4 Polynucleotide Kinase.  DNA should not be precipitated from or dissolved in buffers containing ammonium salts.
  • Protruding 5’-overhangs are more efficiently phosphorylated than blunt- or recessed 5’ overhangs.

References:

  1. Sambrook, J. and Russell, W. (2001) Molecular Cloning: A Laboratory Manual, (3rd ed.), 9.55-9.56, 9.68-9.69.
  2. Sambrook, J. and Russell, W. (2001) Molecular Cloning: A Laboratory Manual, (3rd ed.), 13.16-13.17.
  • Ordering

  • Manuals

  • Resources

Contact your local distributor for pricing
Product Description Size Cat. No.
T4 Polynucleotide Kinase   1,000 U 30061-1
    5,000 U 30061-3
ORDER INFORMATION
未找到相应参数组,请于后台属性模板中添加
暂未实现,敬请期待
暂未实现,敬请期待
上一篇
下一篇