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EconoTaq® DNA Polymerase (separate Mg++)

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EconoTaq® DNA Polymerase (separate Mg++)

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产品名称:

EconoTaq® DNA Polymerase (separate Mg++)

货号
30032-1
规格
1,000 U
存储温度
-20º C
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产品描述

EconoTaq® DNA Polymerase

 

产品说明书

 

  • Great Taq Polymerase performance at a low price.
  • Choice of reaction buffers, with or without MgCl2.
  • Non-proofreading Polymerase
 

 

At -- for 1000 units list price (quantity discounts available), EconoTaq’s low price is coupled with high quality and performance.

QC specifications for EconoTaq are rigorous:

  • Greater than 99% pure by SDS gel electrophoresis (see Figure 1).
  • No detectable DNA contamination as determined by PCR using generic primers.
  • No detectable endonuclease (nicking) activity. Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of supercoiled pBR322 DNA for 16 hours at 70°C results in no detectable conversion to relaxed or linear forms by agarose gel electrophoresis.
  • No detectable exonuclease activity. Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of Hind III-cut lambda DNA for 16 hours at 70°C results in no smearing of bands on agarose gels.

EconoTaq Performance 
As shown in Figures 2 and 3 Lucigen’s EconoTaq DNA Polymerase performs as well as, or better than, more expensive Taq preparations from several other suppliers in routine PCR. EconoTaq is as effective in DNA amplification as another standard PCR enzyme, Tfl DNA polymerase (Figure 4). In this case, the background of non-specific amplification was much lower with EconoTaq (compare “+”and “–“ lanes for EconoTaq and Tfl in Figure 4). EconoTaq DNA Polymerase also offers high lot-to-lot reproducibility and reliability (Figures 2 and 4)

EconoTaq High Purity

EconoTaq Comparison Promega and NEB

Figure 1. High purity of EconoTaq DNA Polymerase (SDS PAGE). Lane 1, broad range molecular weight markers; Lane 2, Lucigen EconoTaq DNA Polymerase

Figure 2. Taq DNA polymerase from Promega and New England Biolabs were compared to Lucigen’s EconoTaq DNA Polymerase (2 different lots) in amplifying the ampicillin gene (0.8 kb) in a pUC19 vector. (–), no DNA. (+), DNA added (40 ng). MW, 1 kb ladder.

EconoTaq Comparison AmpliTaq

Figure 3. EconoTaq vs. AmpliTaq® (Applied Biosystems) DNA polymerase in genotyping. All PCR reactions were performed in a RoboCycler 96 (Stratagene). Hip1 genotyping was performed using the following PCR conditions: 94°C for 1min, 35 cycles of 94°C for 30sec, 62°C for 60sec, 72°C for 90sec, and 72°C for 7min. Shh, Cdo and Gas1 genotyping were performed using the following PCR conditions: 94°C for 2min, 35 cycles of 94°C for 60sec, 65°C for 60sec, 72°C for 90sec, and 72°C for 7min. All PCR reactions contained final concentrations of 1 µM of each primer, 200 µM dNTPs, 1X cresol red loading dye, and 1U of the indicated Taq polymerase. Sequences for all PCR primers have been previously published (references available).
Data courtesy of Dr. Benjamin Allen, Dept. Molecular & Cellular Biology, Harvard University.

EconoTaq Tfl Comparison

Figure 4. PCR amplification was performed under standard conditions using three different lots of EconoTaq DNA Polymerase and buffer, or duplicate reactions with Tfl DNA polymerase and buffer (Promega). Reactions contained primers specific for the 16S ribosomal RNA gene, with Bacillus genomic DNA (+) or no DNA (-) as a template (1450 bp product expected).

Please Note:
Some applications in which Lucigen's EconoTaq DNA Polymerase can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used. The PCR process is the subject of European Patent Nos. 201,184 and 200,262 owned by Hoffman-LaRoche. Those patents expired on March 28, 2006. The corresponding PCR process patents in the United States expired on March 29, 2005. It is the sole responsibility of the buyer to ensure that use of the product does not infringe the patent rights of third parties.

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  • Specifications

  • Manuals

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Contact your local distributor for pricing
Product Description Size Cat. No.
EconoTaq DNA Polymerase (with Mg++)   250 units 30031-0
    1,000 U 30031-1
    5,000 U (5 x 1,000 U) 30031-2
    10,000 U (10 X 1,000 U) 30031-3
EconoTaq DNA Polymerase (separate Mg++)   1,000 U 30032-1
    5,000 U (5 x 1,000 U) 30032-2
    10,000 U (10 X 1,000 U) 30032-3
ORDER INFORMATION
EconoTaq DNA Polymerase is provided with a choice of 10X Reaction Buffer with Mg++ (“with Mg++”); or 10X Reaction Buffer without Mg++ and a separate tube of 25 mM MgCl2 (“separate Mg++”). Please inquire for Bulk pricing!
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