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Bst DNA Polymerase, Exo- 8,000 U/mL

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Bst DNA Polymerase, Exo- 8,000 U/mL

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产品名称:

Bst DNA Polymerase, Exo- 8,000 U/mL

货号
30027-2
规格
10,000 U
存储温度
-20º C
没有此类产品
产品描述

Bst DNA Polymerase, Exonuclease Minus

 

产品说明书

 

  • High strand displacement activity.
  • Used in isothermal amplification and Next Generation Sequencing.
 

High specific activity for DNA amplification and sequencing.

Applications

  • Strand displacement amplification*
  • DNA sequencing through high GC regions (1,2)
  • Rapid sequencing from nanogram amounts of DNA template (3)

Bst DNA Polymerase, Exonuclease Minus, is a recombinant form of the 67 kDa Bacillus stearothermophilus DNA Polymerase protein (large fragment). The enzyme has 5’-3’ polymerase activity and strand displacement activity, but it lacks 3’-5’ exonuclease activity. It also has reverse transcription activity.

Lucigen's Bst DNA Polymerase, Exonuclease Minus, has higher strand displacement activity than that of other suppliers (Figure 1). The enzyme can be used in nucleic acid amplification methods* such as isothermal amplification, whole genome amplification (WGA), and multiple displacement amplification (MDA). It also can be used in Next Generation sequencing.

This enzyme has optimal activity at 65°C. It is suitable for sequencing DNA with high GC content and secondary structures. It is available in concentrations of 8,000 U/ml or 50,000 U/ml.

Bst DNA Polymerase, Exonuclease Minus, is supplied with 10X DNA Polymerase Buffer B, composed of 200 mM Tris-HCl pH 8.8, 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4, and 1.0 % Triton X-100.

Lucigen Bst versus competitors.

Figure 1. Lucigen Bst DNA Polymerase, Exonuclease Minus, possesses greater strand-displacing polymerase activity. M13 single stranded DNA was incubated with or without 8 units of Bst DNA Polymerase(+/- Bst) in reaction buffer supplied by the manufacturer, with or without replication primer (+/- primer) for 30 minutes at 65°C. MW, 1 kb ladder.

Tips for use:

  • Requires 0.1% Triton X-100 for long term storage.
  • Reaction temperatures above 70°C are not recommended.
  • Bst DNA Polymerase cannot be used for thermal cycle sequencing.

Heat Inactivation: 80ºC for 20 min.

References:

  1. Griffin, H. and Griffin, A. (1994) PCR Technology, 228-229.
  2. McClary, J. et al. (1991) J. DNA Sequencing and Mapping, 1, 173-180.
  3. Mead, D.A. et al. (1991) Biotechniques, 11, 76-87.


*Note: 
Some uses for this product may require licenses. Lucigen does not encourage or support the unauthorized or unlicensed use of patented nucleic acid amplification processes for isothermal amplification, whole genome amplification (WGA), multiple displacement amplification (MDA), and Next Generation sequencing. It is the sole responsibility of the buyer to ensure that use of the product does not infringe the patent rights of third parties. If the purchaser is not willing to accept these use limitations, Lucigen Corporation is willing to accept return of the product for a full refund.

  • Ordering

  • Specifications

  • Manuals

  • Resources

Contact your local distributor for pricing
Product Description Size Cat. No.
Bst DNA Polymerase, Exonuclease Minus 8,000 U/ml   200 Units 30027-0
    2,000 U 30027-1
    10,000 (5 x 2,000 Units) 30027-2
Bst DNA Polymerase, Exonuclease Minus 50,000 U/ml   10,000 Units 30028-1
ORDER INFORMATION
Bst DNA Polymerase, Exonuclease Minus is available in two concentrations, 8,000 U/ml and 50,000 U/ml, in a storage buffer of 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, and 50% Glycerol. Also supplied is 10 X DNA Polymerase Buffer B, composed of 200 mM Tris-HCl pH 8.8, 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4,  and 1.0 % Triton X-100.
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