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MasterAmp™ PCR Optimization Kit

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MasterAmp™ PCR Optimization Kit

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产品名称:

MasterAmp™ PCR Optimization Kit

货号
MOS001
规格
20 Temp
存储温度
-20℃
没有此类产品
产品描述

 

Applications

 

  • Simplified PCR optimization with a thermostable DNA polymerase of choice.
  • PCR of difficult or high-GC templates.
  • PCR amplifications up to 20 kb.
  • Multiplex PCR.

For rapid, dependable optimization of PCR, we encourage you to try the FailSafe™ PCR System to obtain the best PCR results with any template up to ~20 kb. However, if you prefer to use another enzyme, EPICENTRE also has two MasterAmp™ PCR Optimization Kits that will help you to obtain the best possible PCR results with thermostable enzymes frequently used in PCR, such as Taq, Pfu, Tth, Tfl,and AmpliTherm™ DNA polymerases. These MasterAmp PCR Optimization Kits contain a series of 12 MasterAmp PCR 2X PreMixes that have been meticulously tested and represent a complete range of PCR conditions frequently encountered. Each MasterAmp 2X PreMix contains all components needed for PCR except for the primers, the template, and the PCR enzyme.

First, perform PCR with your template, primers, and the 12 MasterAmp PreMixes. Select the PreMix that gives optimal results. Then, use the same MasterAmp PCR PreMix to obtain reliable, consistent PCR of the same sequence.

Benefits

  • Fast, simple optimization.
  • Extremely high sensitivity and specificity using the PCR Enhancer Technology.
  • MasterAmp PCR PreMixes contain all components needed for successful PCR.
  • Generates PCR products suitable for both TA cloning and blunt-end cloning.

STEP 1. Find the optimal MasterAmp PCR PreMix. Do PCR with your template, primers and PCR enzyme in the 12 PreMixes.

A. A 75%-GC-rich ApoE gene region was amplified in total human genomic DNA using PreMixes A-L. MasterAmp PCR PreMix K produced optimal results in Step 1.

STEP 2. Use the same MasterAmp PCR PreMix to obtain reliable, consistent PCR of the same sequence.

B. Subsequent PCR of the ApoE gene with the same primer pair and MasterAmp™ PreMix K gave consistent results. Subsequent PCR results are consistent and reliable using the optimal PreMix in Step 1.

MasterAmp™ AmpliTherm DNA Polymerase; MasterAmp PCR Optimization Kits; MasterAmp Taq DNA Polymerase; MasterAmp Tfl DNA Polymerase. Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights (such as 5´ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

EPICENTRE has two MasterAmp PCR Optimization Kits. Select the right kit for your DNA polymerase.

 

Catalog No. Size

MasterAmp™ PCR Optimization Kit WITHOUT Ammonium Sulfate
   MOS001 20 Templates
   MO7201 60 Templates
For MasterAmp™ Taq and AmpliTherm™ DNA Polymerases.
Contains 12 MasterAmp™ PCR PreMixes (A-L), 0.5 ml or 1.5 ml each for 20 or 60 templates, respectively.

MasterAmp™ PCR Optimization Kit WITH Ammonium Sulfate
   MOS02N 20 Templates
   MO751N 60 Templates
For MasterAmp™ Tfl and Tth DNA Polymerases.
Contains 12 MasterAmp™ Ammonium Sulfate PCR PreMixes (AN-LN), 0.5 ml or 1.5 ml each for 20 or 60 templates, respectively.

MasterAmp™ AmpliTherm™ DNA Polymerase
   AT72250N 250 U
Enzyme only. For use with MasterAmp™ PCR PreMixes without ammonium sulfate.

MasterAmp™ Taq DNA Polymerase
   Q82250N 250 U
Enzyme only. For use with MasterAmp™ PCR PreMixes without ammonium sulfate.

MasterAmp™ Tfl DNA Polymerase
   F72250N 250 U
Enzyme only. For use with MasterAmp™ PCR PreMixes with ammonium sulfate.

MasterAmp™ Tth DNA Polymerase
   TTH7225N 250 U
Enzyme only. For use with MasterAmp™ PCR PreMixes with ammonium sulfate.
 

Individual MasterAmp™ 2X PCR PreMixes (listed below):

Product numbers ending in "N" contain ammonium sulfate.


MasterAmp™ 2X PCR PreMix A or AN
   MO7205A 5 ml
   MO755AN 5 ml

MasterAmp™ 2X PCR PreMix B or BN
   MO7205B 5 ml
   MO755BN 5 ml

MasterAmp™ 2X PCR PreMix C or CN
   MO7205C 5 ml
   MO755CN 5 ml

MasterAmp™ 2X PCR PreMix D or DN
   MO7205D 5 ml
   MO755DN 5 ml

MasterAmp™ 2X PCR PreMix E or EN
   MO7205E 5 ml
   MO755EN 5 ml

MasterAmp™ 2X PCR PreMix F or FN
   MO7205F 5 ml
   MO755FN 5 ml

MasterAmp™ 2X PCR PreMix G or GN
   MO7205G 5 ml
   MO755GN 5 ml

MasterAmp™ 2X PCR PreMix H or HN
   MO7205H 5 ml
   MO755HN 5 ml

MasterAmp™ 2X PCR PreMix I or IN
   MO7205I 5 ml
   MO755IN 5 ml

MasterAmp™ 2X PCR PreMix J or JN
   MO7205J 5 ml
   MO755JN 5 ml

MasterAmp™ 2X PCR PreMix K or KN
   MO7205K 5 ml
   MO755KN 5 ml

MasterAmp™ 2X PCR PreMix L or LN
   MO7205L 5 ml
   MO755LN 5 ml

 


MasterAmp™ 10X PCR Enhancer with Betaine
   ME81201 1.5 ml
   ME81205 5 ml
   ME81210 10 ml

 

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