QQ:1443274331

010-88891086,80794780

服务电话:

搜索

 北京市昌平区北清路生命科学园北清创意园2-1-102;  Tel:010-88891086,80794780   Fax:010-80794780 
咨询订购QQ: 1443274331   技术支持QQ: 2074750152   邮件订购:bjzblg@126.com      
www.epibio.com.cn  www.epicentre.cn  www.immunoreagents.cn  www.lucigen.com.cn
版权所有 @北京中北林格科技发展有限公司   
京ICP备:13002510号       网站建设:中企动力 北京

进口试剂

>
>
>
PlasmidMAX™ DNA Isolation Kit

进口试剂

PlasmidMAX™ DNA Isolation Kit

浏览量
产品名称:

PlasmidMAX™ DNA Isolation Kit

货号
PMX51050
规格
50 Preps
存储温度
-20℃
没有此类产品
产品描述

Applications

  • Purification of plasmid clones for DNA sequencing or gene subcloning.

The PlasmidMAX™ DNA Isolation Kit purifies plasmids free from RNA and genomic DNA without the use of columns or hazardous chemicals, using a modified alkaline-lysis procedure. This simple procedure requires only two 1.5-ml tubes, thus avoiding excess plastic waste. The purification method is scaleable to larger volumes, so separate kits are not needed for varying sample sizes. For plasmids 3-40 kb, average sequence reads of >600 bases are obtained from DNA isolated using the PlasmidMAX Kit.

Benefits

  • Simple, rapid miniprep-type procedure.
  • High yields of highly purified plasmid DNA.
  • Scalable to larger volumes.
  • No toxic organic solvents.

 

Figure 1. High-quality sequence data is obtained from plasmid clones purified using the PlasmidMAX™ DNA Isolation Kit.

PlasmidMAX Kit Process

1. Pellet cells. Resuspend in Solution 1.

2. Add Solution 2. Mix.

3. Add Solution 3. Mix.

4. Pellet cell debris and chromosomal DNA.

5. Isopropanol precipitate nucleic acid.

6. Digest RNA with RiboShredder™ RNase.

7. Precipitate and wash plasmid DNA.

 

Figure 2. Agarose gel of plasmid DNAs purified using the PlasmidMAX™ Kit. Lanes 1-3, 4.5 kb plasmid clones; Lane 4, Supercoiled kb ladder.

 

Catalog No. Size

PlasmidMAX™ DNA Isolation Kit
PMX51050 50 Minipreps
Contents: PlasmidMAX™ Solutions 1-3, RiboShredder™ RNase Blend, RiboShredder™ Buffer, Plasmid Precipitation Solution, TE Buffer.

 

未找到相应参数组,请于后台属性模板中添加
暂未实现,敬请期待
暂未实现,敬请期待
上一篇
下一篇