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PlasmidMAX™ DNA Isolation Kit
PlasmidMAX™ DNA Isolation Kit
Applications
- Purification of plasmid clones for DNA sequencing or gene subcloning.
The PlasmidMAX™ DNA Isolation Kit purifies plasmids free from RNA and genomic DNA without the use of columns or hazardous chemicals, using a modified alkaline-lysis procedure. This simple procedure requires only two 1.5-ml tubes, thus avoiding excess plastic waste. The purification method is scaleable to larger volumes, so separate kits are not needed for varying sample sizes. For plasmids 3-40 kb, average sequence reads of >600 bases are obtained from DNA isolated using the PlasmidMAX Kit.
Benefits
- Simple, rapid miniprep-type procedure.
- High yields of highly purified plasmid DNA.
- Scalable to larger volumes.
- No toxic organic solvents.
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Figure 1. High-quality sequence data is obtained from plasmid clones purified using the PlasmidMAX™ DNA Isolation Kit. |
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Figure 2. Agarose gel of plasmid DNAs purified using the PlasmidMAX™ Kit. Lanes 1-3, 4.5 kb plasmid clones; Lane 4, Supercoiled kb ladder. |
| Catalog No. | Size |
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| PlasmidMAX™ DNA Isolation Kit | |
| PMX51050 | 50 Minipreps |
| Contents: PlasmidMAX™ Solutions 1-3, RiboShredder™ RNase Blend, RiboShredder™ Buffer, Plasmid Precipitation Solution, TE Buffer. | |