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MasterAmp™ Extra-Long DNA Polymerase Mix

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MasterAmp™ Extra-Long DNA Polymerase Mix

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产品名称:

MasterAmp™ Extra-Long DNA Polymerase Mix

货号
QU92125
规格
125 U @ 2.5 U/μl
存储温度
-20℃
没有此类产品
产品描述

Applications

  • High-fidelity PCR amplification of sequences up to ~40 kb.

The MasterAmp™ Extra-Long PCR Kit is a complete system for successful and accurate one-step amplification of DNA sequences up to ~40 kb. The kit also contains nine MasterAmp Extra-Long PCR 2X PreMixes with dNTPs, buffer, and varying amounts of MgCl2 and the MasterAmp PCR Enhancer with betaine.* Perform Step 1 (Fig. 1) with each new template and primer set. One PreMix will amplify the long template and give consistent results in all subsequent amplifications (Step 2, Fig. 1).

Benefits

  • Fast optimization; Extra-Long PCR 2X PreMixes with MasterAmp PCR Enhancer, as well as a robust and high-fidelity DNA polymerase mix, allow optimized extra-long PCR (Fig. 2).

 

 

 

STEP 1. Find the optimal MasterAmp™ Extra-Long PCR PreMix. Perform PCR with your template, primers, and the nine PreMixes.

 

STEP 2. Use the same MasterAmp™ Extra-Long PCR PreMix to obtain reliable, consistent PCR of the same sequence.

A. Amplification of a 20-kb region of lambda DNA using MasterAmp™ Extra-Long PCR 2X PreMixes (1-9). MasterAmp™ Extra-Long PCR PreMix 4 produced optimal results.   B. Subsequent PCR of a 20-kb lambda DNA region with the same primer pair and MasterAmp™ PreMix 4 gave consistent results, using the optimal PreMix determined in Step 1.

Figure 1. Two steps to reliable and consistent PCR results.

 

 

Figure 2. Extra-long amplification of lambda DNA using the MasterAmp™ Extra-Long PCR Kit. PCR conditions were optimized and MasterAmp Premix 4 was selected for PCR of 1 ng of lambda DNA in each sample. Results were analyzed on a 0.5% agarose gel. Amplicon sizes were 30 kb (lane 1), 35 kb (lane 2) and 40 kb (lane 3). Lane M, 5-Kb DNA ladder.

*Covered by issued and/or pending patents.

MasterAmp™ Extra-Long PCR Kit; Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser"s own internal research. No other patent rights (such as 5´ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

 

 

 

Catalog No. Concentration Size

MasterAmp™ Extra-Long PCR Kit
   MHF9220   50 Reactions
Includes MasterAmp™ Extra-Long PCR PreMixes 1-9, MasterAmp™ Extra-Long DNA Polymerase Mix, Control Lambda DNA, Primers

MasterAmp™ Extra-Long PCR 2X Premix 1
   MHF925A   5 ml

MasterAmp™ Extra-Long PCR 2X Premix 2
   MHF925B   5 ml

MasterAmp™ Extra-Long PCR 2X Premix 3
   MHF925C   5 ml

MasterAmp™ Extra-Long PCR 2X Premix 4
   MHF925D   5 ml

MasterAmp™ Extra-Long PCR 2X Premix 5
   MHF925E   5 ml

MasterAmp™ Extra-Long PCR 2X Premix 6
   MHF925F   5 ml

MasterAmp™ Extra-Long PCR 2X Premix 7
   MHF925G   5 ml

MasterAmp™ Extra-Long PCR 2X Premix 8
   MHF925H   5 ml

MasterAmp™ Extra-Long PCR 2X Premix 9
   MHF925I   5 ml

MasterAmp™ Extra-Long DNA Polymerase Mix (Enzyme Mix Only)
   QU92125 2.5 U/µl 125 Units
   QU92500 2.5 U/µl 500 Units
   QU9201K 2.5 U/µl 1,000 Units

 

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