pCC1FOS/pEpiFOS-5 Forward Sequencing Primer

Applications

• Preparation of complete and unbiased fosmid libraries that can be maintained at single-copy number.

The EpiFOS™ Fosmid Library Production Kit provides all reagents needed to construct up to 10 complete and unbiased primary fosmid libraries. Fosmid clones constructed in the pEpiFOS-5 Vector cannot be induced to high-copy number, limiting the DNA yields during purification. For this reason, EPICENTRE researchers prefer the use of the CopyControl™ Fosmid Library Construction Kit in the construction of their genomic libraries.

Primers for end-sequencing pEpiFOS-5 clones are available separately.

Benefits

• EpiFOS Vectors are supplied Cloning-Ready: linearized, dephosphorylated, purified, and ready for ligation.
• No need for partial restriction endonuclease digests or pulse-field gel electrophoresis to prepare the genomic DNA for cloning.
• High-efficiency lambda packaging eliminates background and false positives.
• Faster and easier than BAC cloning.

Figure 2. EpiFOS™-5 Fosmid Vector.