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RepliPHI™ Phi29 Polymerase Dilution Buffer

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RepliPHI™ Phi29 Polymerase Dilution Buffer

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产品名称:

RepliPHI™ Phi29 Polymerase Dilution Buffer

货号
RPB04041
规格
1 ml
存储温度
-20℃
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产品描述

RepliPHI™ Phi29 DNA Polymerase (φ29 DNA Polymerase) is a highly processive enzyme with exceptional strand-displacement activity. The enzyme also contains a 3´→5´ exonuclease activity that enables proofreading capability.

Specific Activity: 1 x 106 Units/mg.

Storage Buffer: RepliPHI™ Phi29 DNA Polymerase is supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.5% NP-40, and 0.5% Tween 20.

Unit Definition: One unit of RepliPHI™ Phi29 DNA Polymerase converts 25 pmol of dNTPs into acid-insoluble material in 30 minutes at 30°C under standard assay conditions.

Reaction Buffer: A Reaction Buffer is not supplied with RepliPHI Phi29 DNA Polymerase; however, a suggested Reaction Buffer is 40 mM Tris-HCl (pH 7.5), 50 mM KCl, 10 mM MgCl2, 5 mM (NH4)2SO4, and 4 mM DTT. A 10X Reaction Buffer is supplied with the RepliPHI Phi29 Reagent Sets.

Quality Control: RepliPHI Phi29 DNA Polymerase is free of detectable endonuclease and RNase activities. RepliPHI Phi29 DNA Polymerase is free of detectable DNA contamination.

 

Catalog No. Concentration Size

RepliPHI™ Phi29 DNA Polymerase (enzyme only)
PP031010 1 µg/µl (1,000 U/µl) 10 µg (10,000 Units)
PP040110 0.1 µg/µl (100 U/µl) 10 µg (10,000 Units)

RepliPHI™ Phi29 Reagent Set (includes enzyme, buffer, dNTPs, DTT)
RH031110 Enzyme: 1 µg/µl (1,000 U/µl) Enzyme: 10 µg (10,000 Units)
RH040210 Enzyme: 0.1 µg/µl (100 U/µl) Enzyme: 10 µg (10,000 Units)

RepliPHI™ Phi29 Polymerase Dilution Buffer
RPB04041   1 ml

 

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