EZ-Tn5™ <oriV /KAN-2> Insertion Kit

Applications

• Insertion of priming sites and conversion of single-copy clones to multi-copy-inducible clones for higher yields of BAC or fosmid DNA.

The EZ-Tn5™ <oriV/KAN-2> Insertion Kit* randomly inserts a single EZ-Tn5™ <oriV/KAN-2> Transposon containing an inducible, high-copy origin of replication (oriV) into the target DNA. Thus, the kit integrates CopyControl™ capability into those vectors that lack it. TransforMax™ EPI300™ or EPI300™-T1^R Electrocompetent E. coli are transformed with an aliquot of the reaction and selected for kanamycin resistance encoded by the EZ-Tn5 Transposon. Selected clones are amplified by adding the CopyControl Induction or Autoinduction Solution, and microgram quantities of template DNA can be purified from as little as 1 ml of culture.

Figure 1 (click to enlarge). The process for generating EZ-Tn5™ <oriV/KAN-2> Transposon insertion clones for high yields of DNA and bidirectional sequencing.