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进口试剂
进口试剂
ARTSeq Ribosome Profiling Kit (Mammalian)
ARTSeq Ribosome Profiling Kit (Mammalian)
ARTseq™ Ribosome Profiling Kits
Applications
The ARTseq™ Kit produces RNA-Seq libraries from ribosome-protected mRNA to:
- Investigate translational control.
- Measure gene expression.
- Identify translation start sites.
- Predict protein abundance.
- Investigate translational and co-translational processes in vivo.
Ribosome profiling is a novel technique for investigating translational control. The method, based on deep sequencing of ribosome protected mRNA fragments, provides a "snapshot" of the ribosomes active in a cell at a specific time point. This information can in turn be used to determine what proteins are being actively translated in a cell.
Benefits
- Incorporates a rapid and simplified size-exclusion spin-column method to isolate monsomes.
- Compatible with yeast and mammalian samples.
- ARTseq libraries are compatible with Illumina® Cluster Kits for single read, paired-end and multiplex sequencing using the Illumina GAII, HiSeq and MiSeq sequencers.
- Index-compatible: 12 Index Primers included in the kit.
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Figure 2. Distribution of sequencing reads from ribosome protected RNA or poly(A)+ mRNA samples along a representative yeast transcript (CWP2). A) ARTseq RNA-Seq libraries prepared after Size-exclusion chromatography (SEC) or sucrose gradient centrifugation (Gradient) result in essentially identical distribution of read densities and are enriched for Open Reading Frame (ORF) and devoid of Untranslated sequences (UTR). B) Graphic representation of percent of reads that align to coding, UTR, intron and intergenic regions of the the transcript. |
ARTseq™ Ribosome Profiling Kit - Yeast
RPYSC12116 | 12 Reactions |
ARTseq™ Ribosome Profiling Kit - Mammalian
RPHMR12126 | 12 Reactions |
Ribo-Zero™ Magnetic Kit (Human/Mouse/Rat) (for ARTseq)
MRZH11124 | 24 Reactions |
Ribo-Zero™ Gold Kits (Human/Mouse/Rat) (for ARTseq)
MRZG12324 | 24 Reactions |
MRZG126 | 6 Reactions |
Ribo-Zero™ Magnetic Gold Kit (Yeast) (for ARTseq)
MRZY1324 | 24 Reactions |