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AmpliCap™ SP6 High Yield Message Maker Kit

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AmpliCap™ SP6 High Yield Message Maker Kit

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产品名称:

AmpliCap™ SP6 High Yield Message Maker Kit

货号
AC0706
规格
25 Rxns
存储温度
-20℃
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产品描述

Applications

  • Generate 5´-capped RNA from DNA cloned downstream of a phage SP6 RNA Polymerase promoter for in vitro translation and microinjection.
  • Functional studies of heterogenous nuclear RNAs and viral RNAs.

The AmpliCap™ SP6 High Yield Message Maker Kit is optimized to produce high yields of RNA having a standard m7Gp3G cap on the 5´ end.

Benefits

  • Yield up to 35 µg of RNA from 1 µg of SP6 control template in a 20-µl reaction in 2 hours.
  • Capping efficiencies up to 80%.
  • An optimized m7G[5´]ppp[5´]G Cap/NTP PreMix is provided for ease of use and highest yields of capped RNA transcripts.
  • A separate vial of GTP is provided for efficient production of long, 5´-capped RNA.
  • Formulated to use high concentrations of NTPs that are inhibitory to conventional in vitro transcription kits.
  • RNase inhibitor included.


 

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