AmpliCap™ SP6 High Yield Message Maker Kit

Applications

• Generate 5´-capped RNA from DNA cloned downstream of a phage SP6 RNA Polymerase promoter for in vitro translation and microinjection.
• Functional studies of heterogenous nuclear RNAs and viral RNAs.

The AmpliCap™ SP6 High Yield Message Maker Kit is optimized to produce high yields of RNA having a standard m⁷Gp₃G cap on the 5´ end.

Benefits

• Yield up to 35 µg of RNA from 1 µg of SP6 control template in a 20-µl reaction in 2 hours.
• Capping efficiencies up to 80%.
• An optimized m⁷G[5´]ppp[5´]G Cap/NTP PreMix is provided for ease of use and highest yields of capped RNA transcripts.
• A separate vial of GTP is provided for efficient production of long, 5´-capped RNA.
• Formulated to use high concentrations of NTPs that are inhibitory to conventional in vitro transcription kits.
• RNase inhibitor included.