NTPhos™ Thermolabile Phosphatase

Applications

• Removing 5´ phosphates from modified or unmodified NTPs, NDPs, NMPs, dNTPs, dNDPs, dNMPs, and inorganic pyrophosphate.
• Removing ATP from enzymatic reactions.

NTPhos™ Thermolabile Phosphatase is a novel recombinant enzyme that is highly active in removing phosphate groups from the 5´ position of ribonucleoside- or 2´-deoxyribonucleoside- 5´-triphosphates, diphosphates, or monophosphates. It can be used to remove 5´-phosphate groups from nucleotide substrates following reactions using enzymes such as RNA-dependent or DNA-dependent RNA or DNA polymerases, terminal transferases, or poly(A) polymerases in order to prevent the nucleotides from inhibiting, interfering with, or causing background problems in subsequent downstream reactions.

Following removal of 5´ phosphates from nucleotides, the NTPhos enzyme can be completely and irreversibly heat-inactivated by incubation at 65°C for 15 minutes.

Unit Definition: One unit of NTPhos Thermolabile Phosphatase results in the release of 1 nmol of inorganic phosphate from ATP or dATP in 1 minute at 37°C under standard assay conditions.

Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM zinc acetate, 10 mM MgCl₂, 0.1 M NaCl, and 0.1% Triton® X-100.

Quality Control: NTPhos Phosphatase is free of detectable DNA exo- and endonuclease and RNase activities.