TargetAmp™-Pico Labeling Kit for Illumina® Expression BeadChip

Application

Prepare biotin-antisense RNA (aRNA) for use with Illumina® Expression BeadChip® arrays from minute amounts of RNA. Ideal for samples such laser-capture microdissected (LCM) tissues, flow-sorted cells, or biopsy tissue.

The TargetAmp™-Pico Labeling Kit for Illumina® Expression BeadChip®* is the only kit that can produce micrograms amounts of biotin-aRNA (also called cRNA) from as little as 50 pg of total RNA for use on Illumina Expression BeadChip arrays. The kit uses a significantly improved Eberwine¹ linear RNA amplification method, but incorporates a number of improvements that significantly increase the yields of biotin-aRNA obtained, while reducing the level of nonspecific RNA amplification products.

Benefits

• Linear RNA amplification process ensures preservation of the transcript profile of the sample.
• High correlation with results obtained using the TargetAmp™-Nano Labeling Kit for Illumina Expression BeadChip.
• High yields of biotin-aRNA from as little as 50 pg of total RNA (Table 1).

Figure 1. Genes detected using biotin-aRNA produced by the TargetAmp™-Pico Labeling Kit for Illumina® Expression BeadChip®. Biotin-aRNA was produced from the indicated amount and source of total RNA and hybridized to the Illumina MouseRef-8 Expression BeadChip.

Figure 2. Correlation of expression ratios (click to enlarge). Mouse liver/skeletal muscle expression ratios were obtained with biotin-aRNA produced from 100 ng and 100 pg of total RNA using the TargetAmp-Nano™ and TargetAmp-Pico™ kits, respectively.

References

Van Gelder, R.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 1663.

*TargetAmp™ aRNA Amplification Products are covered by intellectual property licensed to EPICENTRE Technologies Corporation from Johnson & Johnson Pharmaceutical Research & Development, L.L.C., and by intellectual property sublicensed to EPICENTRE Technologies Corporation from Incyte Corporation.

For research use only. Some applications in which this product may be used may be covered by patents or patent applications applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a license, depending upon the particular application and country in which the product is used.