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进口试剂
进口试剂
Colony Fast-Screen™ Kit ( Restriction Screen) 200 Screens
Colony Fast-Screen™ Kit ( Restriction Screen) 200 Screens
Applications
The Colony Fast-Screen™ Kit (Restriction Screen) provides an easy and rapid method to screen the size and orientation of cloned DNA by restriction digests, without overnight cultures or plasmid minipreps (Fig. 1). The Colony Fast-Screen Kit Restricti-Lyse™ solution lyses cells in an environment which is not inhibitory to restriction enzyme activity. Thus, the kit enables the use of any restriction enzyme for restriction analysis. E. coli recombinant colonies can be screened in 25 minutes using the standard protocol (Fig. 2), or in as little as 10 minutes with the accelerated protocol (Fig. 3). Benefits
Notes:
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Figure 1. Schematic overview of the protocol for the Colony Fast- Screen™ Kit (Restriction Screen). |
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Figure 2. Results of the standard 25-minute protocol of the Colony Fast-Screen™ Kit (Restriction Screen).Lane 1, molecular size marker; Lanes 2 to 8, |
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Figure 3. Results of the accelerated 10-minute protocol of the Colony Fast-Screen™ Kit (Restriction Screen). Lanes 1 to 7, |