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Nextera™ DNA Sample Prep Kit (Roche Titanium-compatible)

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Nextera™ DNA Sample Prep Kit (Roche Titanium-compatible)

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产品名称:

Nextera™ DNA Sample Prep Kit (Roche Titanium-compatible)

货号
NT09115
规格
5 Rxns
存储温度
-20℃
没有此类产品
产品描述

Applications

  • Preparation of DNA libraries compatible with Roche 454™ GS FLX and GS FLX Titanium™ next-generation sequencing instruments.

Nextera™ DNA Sample Prep Kits (Roche 454-Compatible) are specifically designed to prepare genomic DNA libraries compatible with Roche 454 GS FLX and GS FLX Titanium instruments. Nextera technology employs in vitro transposition* to simultaneously fragment and tag DNA in a single-tube reaction. The Nextera library preparation method offers significant advantages over existing methods, such as nebulization, sonication, or shearing (Table 1).

Benefits

  • Only 50 ng of starting DNA required.
  • Prepare sequencer-ready libraries in less than 2 hours.
  • Incorporate platform-specific tags and optional bar codes (Fig. 1).
  • Validated on Roche 454 GS FLX and GS FLX Titanium platforms.

*Covered by issued and/or pending patents.

 

Table 1. Library Preparation Comparison
Processing Step Standard (~µg) Nextera™ (50 ng)
Fragmentation ✓ (15-30 min)

Add Nextera™ Enzyme Mix (5 min)

Collection ✓ (15 min)
Concentration ✓ (15 min)
Size Selection ✓ (60 min)
End-Repair ✓ (60 min)
Clean-Up ✓ (15 min)
A-Tailing +/- (30 min)
Adaptor Ligation ✓ (60 min)
Clean-Up ✓ (15 min) ✓ (15 min)
Library Enrichment Variable (~60 min) PCR (~60 min)
Total Time: ~6 Hours <2 Hours

Blue color indicates steps where the sample is transferred to another tube.

Roche/454-Compatible

Figure 1

Figure 1 (click to enlarge). Generation of libraries for Roche 454™ platforms. After fragmenting and tagging genomic DNA with Nextera™ Enzyme Mix, four-primer limited-cycle PCR is performed which adds Roche 454-compatible adaptor sequences (blue and orange). Optional bar codes (triangle) can be added between the upstream emulsion PCR (emPCR) adaptor (blue) and the transposon end (gray).

 

For additional information on Nextera™ technology, please visit: www.epibio.com/nextera

For custom/bulk requests, please contact us at: Nextera@EpiBio.com

 

Catalog No. Size

Nextera™ DNA Sample Prep Kit (Roche Titanium-Compatible)
NT09115 5 Reactions
NT091120 20 Reactions
NT0911-50 50 Reactions
NT0911-96 96 Reactions

Contents: Nextera Enzyme Mix (Roche Ti-Compatible), 5X Nextera Reaction Buffer (HMW), 50X Nextera Primer Cocktail (Roche Ti-Compatible), 50X Nextera Adaptor 1 (Roche Ti-Compatible), Nextera Control DNA, and 2X Nextera PCR Buffer.

*Note: The Nextera™ PCR Enzyme (see below) must be purchased separately when using any of the Nextera™ DNA Sample Prep Kits.


Nextera™ DNA Sample Prep Kit (Roche FLX-Compatible)*
FL09115 5 Reactions
FL091120 20 Reactions

Contents: Nextera Enzyme Mix (Roche FLX-Compatible), 5X Nextera Reaction Buffer (HMW), 50X Nextera Primer Cocktail (Roche FLX-Compatible), 50X Nextera Adaptor 1 (Roche FLX-Compatible), Nextera Control DNA, and 2X Nextera PCR Buffer.

*Note: The Nextera™ PCR Enzyme (see below) must be purchased separately when using any of the Nextera™ DNA Sample Prep Kits.


Nextera™ PCR Enzyme (required)
EM091120 20 Reactions
EM091150 50 Reactions
EM0911-96 96 Reactions

Nextera™ Bar Codes (optional)
FLBC0950 12 Bar Codes (Roche FLX-Compatible)
NTBC0950 12 Bar Codes (Roche Titanium-Compatible)
Note: The bar coding kits are optional. Each kit contains 12 bar codes, and each bar code can be used to prepare up to 50 bar-coded libraries.

 

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