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Nextera™ DNA Sample Prep Kit (Roche FLX-compatible)

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Nextera™ DNA Sample Prep Kit (Roche FLX-compatible)

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产品名称:

Nextera™ DNA Sample Prep Kit (Roche FLX-compatible)

货号
FL091120
规格
20 Rxns
存储温度
-20℃
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产品描述

Applications

  • Preparation of DNA libraries compatible with Roche 454™ GS FLX and GS FLX Titanium™ next-generation sequencing instruments.

Nextera™ DNA Sample Prep Kits (Roche 454-Compatible) are specifically designed to prepare genomic DNA libraries compatible with Roche 454 GS FLX and GS FLX Titanium instruments. Nextera technology employs in vitro transposition* to simultaneously fragment and tag DNA in a single-tube reaction. The Nextera library preparation method offers significant advantages over existing methods, such as nebulization, sonication, or shearing (Table 1).

Benefits

  • Only 50 ng of starting DNA required.
  • Prepare sequencer-ready libraries in less than 2 hours.
  • Incorporate platform-specific tags and optional bar codes (Fig. 1).
  • Validated on Roche 454 GS FLX and GS FLX Titanium platforms.

*Covered by issued and/or pending patents.

 

Table 1. Library Preparation Comparison
Processing Step Standard (~µg) Nextera™ (50 ng)
Fragmentation ✓ (15-30 min)

Add Nextera™ Enzyme Mix (5 min)

Collection ✓ (15 min)
Concentration ✓ (15 min)
Size Selection ✓ (60 min)
End-Repair ✓ (60 min)
Clean-Up ✓ (15 min)
A-Tailing +/- (30 min)
Adaptor Ligation ✓ (60 min)
Clean-Up ✓ (15 min) ✓ (15 min)
Library Enrichment Variable (~60 min) PCR (~60 min)
Total Time: ~6 Hours <2 Hours

Blue color indicates steps where the sample is transferred to another tube.

Roche/454-Compatible

Figure 1 (click to enlarge). Generation of libraries for Roche 454™ platforms. After fragmenting and tagging genomic DNA with Nextera™ Enzyme Mix, four-primer limited-cycle PCR is performed which adds Roche 454-compatible adaptor sequences (blue and orange). Optional bar codes (triangle) can be added between the upstream emulsion PCR (emPCR) adaptor (blue) and the transposon end (gray).

For additional information on Nextera™ technology, please visit: www.epibio.com/nextera

For custom/bulk requests, please contact us at: Nextera@EpiBio.com

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