QQ：1443274331

010-88891086，80794780

服务电话：

搜索

北京市昌平区北清路生命科学园北清创意园2-1-102; Tel：010-88891086，80794780 Fax：010-80794780
咨询订购QQ: 1443274331 技术支持QQ: 2074750152 邮件订购：bjzblg@126.com
www.epibio.com.cn www.epicentre.cn www.immunoreagents.cn www.lucigen.com.cn
版权所有 @北京中北林格科技发展有限公司京ICP备：13002510号网站建设：中企动力北京

进口试剂

Nextera™ Bar Codes (Illumina-compatible)

进口试剂

Nextera™ Bar Codes (Illumina-compatible)

浏览量：

产品名称:

Nextera™ Bar Codes (Illumina-compatible)

货号

GABC0950

规格

12 Bar Codes

存储温度

-20℃

产品描述

Applications

Preparation of DNA libraries compatible with Illumina Solexa® next-generation sequencing instruments.

The Nextera™ DNA Sample Prep Kit (Illumina-Compatible) is specifically designed to prepare genomic DNA libraries compatible with Illumina Solexa GAI and GAII instruments. Nextera technology employs in vitrotransposition* to simultaneously fragment and tag DNA in a single-tube reaction. The Nextera library preparation method offers significant advantages over existing methods, such as nebulization, sonication, or shearing (Table 1).

Benefits

Only 50 ng of starting DNA required.
Prepare sequencer-ready libraries in less than 2 hours.
Incorporate platform-specific tags and optional bar codes (Fig. 1).
Validated on Illumina Solexa GAI and GAII instruments.

*Covered by issued and/or pending patents.

Table 1. Library Preparation Comparison
Processing Step	Standard (~µg)	Nextera™ (50 ng)
Fragmentation	✓ (15-30 min)	Add Nextera™ Enzyme Mix (5 min)
Collection	✓ (15 min)
Concentration	✓ (15 min)
Size Selection	✓ (60 min)
End-Repair	✓ (60 min)
Clean-Up	✓ (15 min)
A-Tailing	+/- (30 min)
Adaptor Ligation	✓ (60 min)
Clean-Up	✓ (15 min)	✓ (15 min)
Library Enrichment	Variable (~60 min)	PCR (~60 min)
Total Time:	~6 Hours	<2 Hours

Blue color indicates steps where the sample is transferred to another tube.

Illumina/Solexa-Compatible

Figure 1 (click to enlarge). Generation of libraries for Illumina Solexa® platforms. Target DNA is fragmented and tagged with Nextera™ Enzyme Mix containing ends appended with sequencing primer sites (blue and orange). Limited-cycle PCR with a four-primer reaction adds bridge PCR (bPCR) compatible adaptors (purple and pink) to the core sequencing library. Optional bar codes (triangle) can be added between the downstream bPCR adaptor (pink) and the core sequencing library adaptor (orange). Alternative sequencing primers are required for the Illumina Solexa-compatible libraries: Read 1 Primer (blue/gray arrow); Read 2 Primer (orange/gray arrow); and Index Read Primer (gray/orange arrow).

For additional information on Nextera™ technology, please visit: www.epibio.com/nextera

For custom lk requests, please contact us at: Nextera@EpiBio.com

BACMAX96™ DNA Purification Kit with Plastic Consumables

BuccalAmp™ DNA Extraction Kit with MasterAmp™ Brush Soft Pack