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进口试剂

进口试剂

TE Buffer

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产品名称:

TE Buffer

货号
MTE0970
规格
70 ml
存储温度
+4℃
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产品描述

Applications

Purification of TNA, DNA, or RNA for many applications, including PCR, RT-PCR, cloning, sequencing, Northern and Southern blotting, restriction analysis, and genomic library preparation.

The MasterPure™ Complete DNA and RNA Purification Kit uses a novel technology that enables efficient purification of intact total nucleic acid (TNA), DNA, or RNA from every type of biological material (Table 1 and Fig. 1).

The gentle salt-precipitation protocol permits rapid purification of high-molecular-weight nucleic acids from a single sample or many samples that are processed simultaneously (Fig. 4). High yields of nucleic acids are obtained using MasterPure Kits (Table 2), and yields and purity are highly consistent for different samples of the same material (Fig. 2). The resultant nucleic acids have an A260/A280 ratio of 1.8-2.0, indicating that they are substantially free of protein. The TNA, DNA, and/or RNA purified from different samples can be used directly in many applications. For example, the sensitivity of PCR or RT-PCR assays is often improved if the template is obtained using a MasterPure Purification Kit.

 

Benefits

  • Purify TNA in 30 minutes without cumbersome columns.
  • A260/A280 ratios consistently between 1.8 and 2.0.
  • Recover >90% of theoretical DNA yield.
  • No phenol, chloroform, or other caustic solvents.
  • Each kit component is also available separately in a 10X package size.
  • Low- and high-molecular-weight nucleic acids are recovered.

Reference

  1. Schanke, J. and Watson, J. (1998) EPICENTRE Forum 5(2), 12.

Figure 1 (click to enlarge). Overview of the MasterPure™ Complete Kit protocol.

Figure 2. Comparison of PCR sensitivity using templates obtained using the MasterPure™ Complete DNA and RNA Purification Kit versus a spin-column kit. PCR amplification was performed after extraction from serial dilutions of E. coli cells ranging from 2 x 107 (lanes 1) to 200 (lanes 6). Lane M, DNA marker; lane 7, negative control.

Figure 3 (click to enlarge). DNA, RNA, and total nucleic acid (TNA) purified from diverse cell sources using the MasterPure™ Complete Kit. M = kb ladder.

 
Targets Samples Extracted

Human & mammalian RNA & genomic DNA
HIV
HCV
RSV
HPV
Yeast
Enterovirus
B. pertussis
E. coli
M. tuberculosis
Streptococcus mutans
Soy (tofu)
Maize
Insect tissues

Plasma
Whole blood
Guthrie cards
Buccal cells
Liver
Mouse tail
Kidney
Saliva
Urine
Sputum
Tissue-culture cell lines
Cervical cells
Paraffin-embedded tissues

Table 1. Examples of targets and samples analyzed using MasterPure™ DNA and RNA Purification Kits. Using an extremely simple salt-precipitation protocol, TNA, DNA, or RNA can be isolated from virtually any source in less than 1 hour.

Sample Sample Size TNA µg DNA µg RNA µg
HeLa/HL60 cells 1 X 106 cells 10-30 3-12 7-15
Tissues
Liver 5 mg 33-42 5-10 13-25
Brain 5 mg 9-13 6-9 4-11
Heart 5 mg 6-10 4-7 4-5
Kidney 5 mg 10-17 3-8 14-17
Thymus 5 mg 15-30 6-12 9-18
Blood 200 µl 3-10 3-9  
Buffy coat 300 µl 40-55 40-55 3-6
Mouse tail 0.5 cm 25-30 9-11  
E. coli 3.5 x 106 cells 2.5-2.8 1.3-1.6 1.6-1.8
S. mutans 1.5 ml 0.9    

Yeast*

(S. cerevisiae)

2.2 x 106 cells     11-18
1.1 x 107 cells     70-78

*For extraction of yeast DNA, the MasterPure™ Yeast DNA Purification Kit is recommended.

Table 2. Example of yields of nucleic acids obtained using MasterPure™ Complete DNA and RNA Purification Kits.

Figure 4. Consistent purification of DNA, TNA, and RNA. Five different genomic DNA (lanes 1-5), TNA (lanes 6-10), and total cellular RNA (lanes 11-15) samples were isolated from frozen bovine liver with the MasterPure™ Complete DNA and RNA Purification Kit. Lane M, Kilobase

 

 

Catalog No. Concentration Size

MasterPure™ Complete DNA and RNA Purification Kit
   MC89010   5/10 Purifications*
   MC85200   200 Purifications*
For isolating TNA, DNA, or RNA.

Contents: Red Cell Lysis Solution, Tissue and Cell Lysis Solution, MPC Protein Precipitation Reagent, 2X T&C Lysis Solution, TE Buffer**, RNase A, RNase-Free DNase I, Proteinase K, 1X DNase Buffer, RiboGuard™ RNase Inhibitor.

*Note: Cat. # MC89010 contains reagents for 10 TNA, 10 DNA, or 5 RNA purifications and Cat. # MC85200 contains reagents for 200 TNA, 200 DNA, or 100 RNA purifications.


MasterPure™ DNA Purification Kit (for isolating TNA or DNA)
   MCD85201   200 Purifications

Contents: Red Cell Lysis Solution, Tissue and Cell Lysis Solution, MPC Protein Precipitation Reagent, 2X T&C Lysis Solution, TE Buffer**, RNase A, Proteinase K.

**Not provided with the 10-purification kit (Cat. # MC89010).


MasterPure™ Individually Packaged Bulk Reagents (10X Package Size)
The following reagents are designed for use with the MasterPure™ kit protocols only.

Red Cell Lysis Solution
   MRC0912H   1200 ml

Tissue & Cell Lysis Solution
   MTC096H   600 ml

MPC Protein Precipitation Solution
   MMP03750   50 ml
   MMP095H   500 ml

TE Buffer
   MTE0970   70 ml

2X T&C Lysis Buffer
   MTC085H   500 ml

Proteinase K
   MPRK092 50 µg/µl 2 ml

DNase I, RNase-Free
   D9905K 1 U/µl 5 ml

1X DNase Buffer
   MBD092H   200 ml

RNase A
   MRNA092 5 µg/µl 2 ml

 

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