MasterAmp™ Extra-Long DNA Polymerase Mix

Applications

• High-fidelity PCR amplification of sequences up to ~40 kb.

The MasterAmp™ Extra-Long PCR Kit is a complete system for successful and accurate one-step amplification of DNA sequences up to ~40 kb. The kit also contains nine MasterAmp Extra-Long PCR 2X PreMixes with dNTPs, buffer, and varying amounts of MgCl₂ and the MasterAmp PCR Enhancer with betaine.* Perform Step 1 (Fig. 1) with each new template and primer set. One PreMix will amplify the long template and give consistent results in all subsequent amplifications (Step 2, Fig. 1).

Benefits

• Fast optimization; Extra-Long PCR 2X PreMixes with MasterAmp PCR Enhancer, as well as a robust and high-fidelity DNA polymerase mix, allow optimized extra-long PCR (Fig. 2).

STEP 1. Find the optimal MasterAmp™ Extra-Long PCR PreMix. Perform PCR with your template, primers, and the nine PreMixes.			STEP 2. Use the same MasterAmp™ Extra-Long PCR PreMix to obtain reliable, consistent PCR of the same sequence.
	A. Amplification of a 20-kb region of lambda DNA using MasterAmp™ Extra-Long PCR 2X PreMixes (1-9). MasterAmp™ Extra-Long PCR PreMix 4 produced optimal results.			B. Subsequent PCR of a 20-kb lambda DNA region with the same primer pair and MasterAmp™ PreMix 4 gave consistent results, using the optimal PreMix determined in Step 1.

Figure 1. Two steps to reliable and consistent PCR results.

*Covered by issued and/or pending patents.

MasterAmp™ Extra-Long PCR Kit; Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser"s own internal research. No other patent rights (such as 5´ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.