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TargetAmp™ Nano-g™ Biotin-aRNA Labeling Kit for the Illumina® System
TargetAmp™ Nano-g™ Biotin-aRNA Labeling Kit for the Illumina® System
由于生产线调整,自2017年1月1日起,此款产品转至Lucigen™品牌销售, (仅品牌变化,产品外包装变化,本身没有改变,老客户可以继续使用,实验不受影响)
Applications
The TargetAmp™ Nano-g™ Biotin-aRNA Labeling Kit for the Illumina® System* produces biotin-aRNA (also called cRNA) for use with Illumina gene expression systems, such as the Illumina Expression BeadChip™ arrays (Fig. 1). TargetAmp Nano-g Kit reactions have been optimized to minimize sample size, and maximize biotin-aRNA yield and signal intensity. A TargetAmp Nano-g Kit reaction produces microgram amounts of biotin-labeled aRNA from as little as 25 ng of input total cellular RNA (Table 1) using an easy linear RNA amplification process. The biotin-aRNA produced by a TargetAmp Nano-g Kit reaction from 100 ng of input total RNA has been shown to detect more transcripts than biotin-aRNA produced by a competitive kit from 400 ng of input total RNA (Fig. 2). Benefits
Table 1. The TargetAmp™ Nano-g™ Kit produces microgram amounts of biotin-aRNA from as little as 25 ng of input total RNA. Results represent the average of multiple experiments.
a. Approximately 2% of HeLa total RNA is poly(A) RNA. Table 2. A TargetAmp™ Nano-g™ Kit procedure is faster and easier to perform than a competitive kit.
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*TargetAmp™ aRNA Amplification Products are covered by intellectual property licensed to EPICENTRE Technologies Corporation from Johnson & Johnson Pharmaceutical Research & Development, L.L.C., and by intellectual property sublicensed to EPICENTRE Technologies Corporation from Incyte Corporation.
For research use only. Some applications in which this product may be used may be covered by patents or patent applications applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a license, depending upon the particular application and country in which the product is used.
Catalog No. | Size |
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TargetAmp™ Nano-g™ Biotin-aRNA Labeling Kit for the Illumina® System | |
TAN07924 | 24 Reactions |
TAN091096 | 96 Reactions |
Contents: TargetAmp™ Nano-g™ T7-Oligo(dT) Primer, TargetAmp™ Nano-g™ 1st-Strand cDNA PreMix, TargetAmp™ Nano-g™ 2nd-Strand cDNA PreMix, TargetAmp™ Nano-g™ 2nd-Strand DNA Polymerase, TargetAmp™ Nano-g™ T7 RNA Polymerase, TargetAmp™ Nano-g™ T7 Transcription Buffer, TargetAmp™ Nano-g™ NTP PreMix, TargetAmp™ Nano-g™ UTP / Biotin-UTP, RNase-Free DNase I, DTT, HeLa Total RNA Control, RNase-Free Water. |