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Epicentre Ribo-Zero™系列新产品去rRNA试剂盒(植物种子,根系)Ribo-Zero™ rRNA Removal Kit

浏览次数: 日期:2011-6-10

 

Ribo-Zero™ rRNA Removal Kit (Plant Leaf) Ribo-Zero™ rRNA Removal Kit (Plant Seed/Root)

The Ribo-Zero™ rRNA Removal Kit (Plant Leaf) and Ribo-Zero™ rRNA Removal Kit (Plant Seed/Root) utilize an improved hybridization-capture technology that removes virtually all the cytoplasmic (nuclear-encoded) and chloroplast ribosomal RNA (rRNA) from intact and partially degraded plant leaf, seed, and root total RNA.

Benefits

  • Removes >99.5% of both cytoplasmic and chloroplast rRNA from intact and partially degraded plant leaf, seed, and root total RNA preparations.
  • Single-pass, 1-hour process yields RNA sufficiently depleted of rRNA for RNA-Seq, random-primed cDNA synthesis, and other applications.
  • Recovers mRNAs, as well as other non-rRNAs that lack a poly(A) tail.
  • Recovers both the 5´- and 3´-end fragments of mRNAs in a partially degraded RNA sample.

Table 1. Removal of plant cytoplasmic and chloroplast rRNA using the Ribo-Zero™ rRNA Removal Kit. One to five micrograms of the indicated RNA were treated using the appropriate Ribo-Zero Kit. The amount of residual rRNA in the Ribo-Zero-treated RNA was determined by random-primed reverse transcription-qPCR (qRT-PCR) using PCR primers to the small rRNAs and to multiple regions of the large rRNAs. Percent rRNA removal was calculated from rRNA-specific ΔCt values, normalized to those of the EF-1α transcript.

 

Input RNA Sample Input Amounts % rRNA Removal (Nuclear) % rRNA Removal (Chloroplast)
25S 18S 5.8S 23S 16S 5S 4.5S
Arabidopsis thaliana leaf 1-5 µg >99.99% >99.96% >99.94% >99.99% >99.98% >99.97% >99.94%
Soybean root 1-5 µg >99.99% >99.95% >99.99% >99.98% >99.97% >99.75% >99.95%
Soybean seed 1-5 µg >99.99% >99.99% >99.97% >99.98% >99.98% >99.85% >99.95%

Figure 1 (click to enlarge). Agilent Bioanalyzer analysis of RNA treated with the Ribo-Zero™ (Plant Leaf) Kit. Bioanalyzer (RNA600 Pico Chip) profiles of partially degraded Arabidopsis thaliana RNA (BioChain®, Hayward, CA) before (red) and after (blue) a Ribo-Zero™ (Plant Leaf) reaction. The Ribo-Zero-treated RNA was purified using an RNA Clean & Concentrator-5 Column (Zymo Research) following the Zymo "General Procedure, total RNA (>17nt)".

Figure 2 (click to enlarge). Agilent Bioanalyzer analysis of RNA treated with the Ribo-Zero™ (Plant Seed/Root) Kit. Bioanalyzer profile (RNA600 Pico Chip) of soy root RNA before (green) and after (blue and red, from 1 µg and 5 µg total RNA, respectively) a Ribo-Zero (Plant Seed/Root) reaction. The Ribo-Zero-treated RNA was purified using an RNA Clean & Concentrator™ Column (Zymo Research) following the Zymo "General Procedure, total RNA (>17 nt)".


 

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 Catalog No. Size

Ribo-Zero™ rRNA Removal Kit (Plant Leaf)
   RZPL11016 6 Reactions

Ribo-Zero™ rRNA Removal Kit (Plant Seed/Root)
   RZSR11036 6 Reaction

 

 系列试剂还有:

 

Ribo-Zero™ rRNA Removal Kit (Gram-Negative Bacteria)
   RZNB1056 6 Reactions

 

Ribo-Zero™ rRNA Removal Kit (Gram-Positive Bacteria)
   RZPB10106 6 Reactions

 

Ribo-Zero™ rRNA Removal Kit (Human/Mouse/Rat) Low Input
   RZH1086 6 Reactions

 

Ribo-Zero™ rRNA Removal Kit (Human/Mouse/Rat)
   RZH1046 6 Reactions
   RZH110424 24 Reactions

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