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Biosearch technologies lucigen感受态细胞选择指南

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Biosearch technologies lucigen感受态细胞选择指南

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【摘要】:
感受态细胞选择指南   Blue-whitecapablPhageT1resistantEndA-/RecARestriction-deficient(RMS)ClonemethylatedDNAInducibleplasmidcopynumberF’episomeAntibioticresistanceElectrocompetent:efficiencyChemicallycompetent:
看看

 

Blue-white capabl

Phage T1 resistant

End A -/RecA

Restriction-deficient (RMS)

Clone methylated DNA

Inducible plasmid copy number

F’episome

Antibiotic resistance

Electrocompetent:efficiency

Chemically competent:efficiency

常规克隆及文库构建

 

 

 

E. cloni® 10G (Classic)

Ø

Ø

Str

5 x109

106-109

E. cloni 10G (Elite)

Ø

Ø

Str

2 x1010

-

E. cloni 10G (Supreme)

Ø

Ø

Str

4 x1010

-

E.cloni 10G F’(Elite)

Ø

Str

2 x1010

-

E. cloni 5-alpha

Ø

Ø

Ø

Ø

-

-

1 x108

不稳定的插入物或有毒基因

 

 

 

 

 

 

 

 

 

 

Copycutter™ EPI400™

Ø

Str

1 x1010

1 x107

大片段,plasmids和fosmids质粒

 

 

 

 

 

 

 

 

 

 

TransforMax™ EC100™

Ø

Ø

Ø

Str

1 x1010

5 x108

TransforMax EPI300™

Ø

a

Ø

Str

1 x1010

5 x108

TransforMax EPI300™-T1R

a

Ø

Str

1 x1010

 

BAC克隆

 

 

 

 

 

 

 

 

 

 

BAC-Optimised Replicator™ v2.0

Ø

a

Ø

Amp, Str

1 x1010

-

E. cloni BAC-Optimised 10G

Ø

Ø

Ø

Str

1 x1010

-

BigEasy™-TSA

b

c

Ø

Amp, Str

2 x1010

-

噬菌体展示

 

 

 

 

 

 

 

 

 

 

TG1

Ø

Ø

Ø

d

Ø

-

4 x1010

-

ER2738

Ø

Ø

d

Ø

Tet

2 x1010

-

SS320 (MC1061 F’)

Ø

Ø

Ø

e

Ø

Tet, Str

4 x1010

-

MC1061 F-

Ø

Ø

Ø

Ø

e

Ø

Ø

Str

4 x1010

-

CRISPR sgRNA文库及慢病毒质粒克隆

 

 

 

 

 

 

 

 

 

 

Endura™

Ø

Ø

f

Ø

g

Ø

Ø

Str

1 x1010

 

Site-directed mutagenesis

 

 

 

 

 

 

 

 

 

 

CJ236

Ø

Ø

Ø

Ø

Ø

Ø

Cam

1 x109

-

 


Amp, ampicillin; Cam, chloramphenicol; Str, streptomycin; Tet, tetracycline
 
a: Requires OriV vectors (e.g., CopyRight® and pCCFOS)
b: Requires IPTG
c: Only with pJAZZ® vectors
d: mcrB- only; not suitable for cloning eukaryotic genomic DNA
e: mcrA- mcrB-; not suitable for cloning eukaryotic genomic DNA
f: RecA13 only
g: mcrB- mrr-; not suitable for cloning eukaryotic genomic DNA
 

 

 

Primary promoter

Background expression control

Low endotoxin

Antibiotic resistance

Electrocompetent:efficiency

Chemically competent:efficiency

常规蛋白表达

 

 

 

 

 

 

E. cloni EXPRESS BL21(DE3)

T7

Ø

Ø

-

5 x 109

1 x 107

Hl-Control™ BL21(DE3)

T7

Ø

Gen

-

1 x 107

Hl-Control™ 10G

Plac,Ptac,Ptrc,T5lac,rhaPBAD

Ø

Gen, Str

-

1 x 109

ClearColi® BL21(DE3)

      T7

Ø

-

1 x 109

-

带毒性蛋白表达

 

 

 

 

 

 

OverExpress™ C41(DE3)

T7

Ø

Ø

-

1 × 109

1 × 106

OverExpress C41(DE3) pLysS

T7

Ø

Cam

-

1 × 106

OverExpress C43(DE3)

T7

Ø

Ø

-

1 × 109

1 × 106

OverExpress C43(DE3) pLysS

T7

Ø

Cam

-

1 × 106