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技术资料
技术资料
Biosearch technologies lucigen感受态细胞选择指南
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Blue-white capabl |
Phage T1 resistant |
End A -/RecA |
Restriction-deficient (RMS) |
Clone methylated DNA |
Inducible plasmid copy number |
F’episome |
Antibiotic resistance |
Electrocompetent:efficiency |
Chemically competent:efficiency |
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常规克隆及文库构建 |
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E. cloni® 10G (Classic) |
√ |
√ |
√ |
√ |
√ |
Ø |
Ø |
Str |
≥5 x109 |
106-109 |
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E. cloni 10G (Elite) |
√ |
√ |
√ |
√ |
√ |
Ø |
Ø |
Str |
≥2 x1010 |
- |
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E. cloni 10G (Supreme) |
√ |
√ |
√ |
√ |
√ |
Ø |
Ø |
Str |
≥4 x1010 |
- |
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E.cloni 10G F’（Elite) |
√ |
√ |
√ |
√ |
√ |
Ø |
√ |
Str |
≥2 x1010 |
- |
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E. cloni 5-alpha |
√ |
√ |
√ |
Ø |
Ø |
Ø |
Ø |
- |
- |
≥1 x108 |
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不稳定的插入物或有毒基因 |
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Copycutter™ EPI400™ |
√ |
√ |
√ |
√ |
√ |
√ |
Ø |
Str |
≥1 x1010 |
≥1 x107 |
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大片段,plasmids和fosmids质粒 |
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TransforMax™ EC100™ |
√ |
Ø |
√ |
√ |
√ |
Ø |
Ø |
Str |
≥1 x1010 |
≥5 x108 |
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TransforMax EPI300™ |
√ |
Ø |
√ |
√ |
√ |
a |
Ø |
Str |
≥1 x1010 |
≥5 x108 |
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TransforMax EPI300™-T1R |
√ |
√ |
√ |
√ |
√ |
a |
Ø |
Str |
≥1 x1010 |
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BAC克隆 |
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BAC-Optimised Replicator™ v2.0 |
√ |
Ø |
√ |
√ |
√ |
a |
Ø |
Amp, Str |
≥1 x1010 |
- |
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E. cloni BAC-Optimised 10G |
√ |
Ø |
√ |
√ |
√ |
Ø |
Ø |
Str |
≥1 x1010 |
- |
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BigEasy™-TSA |
b |
√ |
√ |
√ |
√ |
c |
Ø |
Amp, Str |
≥2 x1010 |
- |
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噬菌体展示 |
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TG1 |
√ |
Ø |
Ø |
Ø |
d |
Ø |
√ |
- |
≥4 x1010 |
- |
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ER2738 |
√ |
√ |
Ø |
Ø |
d |
Ø |
√ |
Tet |
≥2 x1010 |
- |
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SS320 (MC1061 F’) |
√ |
Ø |
Ø |
Ø |
e |
Ø |
√ |
Tet, Str |
≥4 x1010 |
- |
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MC1061 F- |
Ø |
Ø |
Ø |
Ø |
e |
Ø |
Ø |
Str |
≥4 x1010 |
- |
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CRISPR sgRNA文库及慢病毒质粒克隆 |
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Endura™ |
Ø |
Ø |
f |
Ø |
g |
Ø |
Ø |
Str |
≥1 x1010 |
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Site-directed mutagenesis |
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CJ236 |
Ø |
Ø |
Ø |
Ø |
Ø |
Ø |
√ |
Cam |
≥1 x109 |
- |
Amp, ampicillin; Cam, chloramphenicol; Str, streptomycin; Tet, tetracycline
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Primary promoter |
Background expression control |
Low endotoxin |
Antibiotic resistance |
Electrocompetent:efficiency |
Chemically competent:efficiency |
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常规蛋白表达 |
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E. cloni EXPRESS BL21(DE3) |
T7 |
Ø |
Ø |
- |
≥5 x 109 |
≥1 x 107 |
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Hl-Control™ BL21(DE3) |
√ |
Ø |
Gen |
- |
≥1 x 107 |
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Hl-Control™ 10G |
Plac,Ptac,Ptrc,T5lac,rhaPBAD |
√ |
Ø |
Gen, Str |
- |
≥1 x 109 |
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ClearColi® BL21(DE3) |
T7 |
Ø |
√ |
- |
≥1 x 109 |
- |
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带毒性蛋白表达 |
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OverExpress™ C41(DE3) |
T7 |
Ø |
Ø |
- |
≥1 × 109 |
≥1 × 106 |
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OverExpress C41(DE3) pLysS |
T7 |
Ø |
Cam |
- |
≥1 × 106 |
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OverExpress C43(DE3) |
T7 |
Ø |
Ø |
- |
≥1 × 109 |
≥1 × 106 |
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OverExpress C43(DE3) pLysS |
T7 |
√ |
Ø |
Cam |
- |
≥1 × 106 |