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感受态细胞选购指引

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感受态细胞选购指引

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【摘要】:
感受态细胞选择指南

Lucigen offers the best performance, value, and convenience in competent cells for cloning and expression. Our Chemically Competent Cells offer an unmatched value for routine cloning, and our Electrocompetent Cells provide the highest possible efficiency for difficult cloning and library construction.

See our Custom Competent Cell Services page to learn how you can receive the cells you need when you need them. Custom dispensing is available in tubes or 96-well plates, for your strain or ours.

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General Cloning & Library Construction

E. cloni® 10G Chemically Competent Cells »Clone, subclone and propagate plasmids with heat-shock transformation. Recommended for transformation of Gibson Assembly® reactions.

TransforMax™ EC100™ Electrocompetent  and Chemically Competent E. coli » Clone and subclone with high efficiency recA, endA strain.

E. cloni® 10G and 10GF' Electrocompetent Cells » Construct libraries, perform general cloning, product ssDNA.

E. cloni® 5-alpha Chemically Competent Cells » Clone, subclone. Transform using heat shock.

Phage Display Library Applications

Phage Display Competent Cells » Create large phage display libraries. Speed discovery.

CRISPR, Large or Difficult Fragment Cloning

Endura™ Competent Cells » Maintain unstable clones and propagate plasmids. Ideal for GeCKO CRISPR library transformation.

TransforMax™ EPI300™ Electrocompetent and Chemically Competent E. coli » Clone large inserts, control copy number of CopyControl™ vectors. EPI300 Electrocompetent cells are recommended for electroporation of Gibson Assembly® reactions.

CopyCutter™ EPI400™ Electrocompetent and Chemically Competent E. coli » Clone unstable and toxic sequences with high efficiency.

TransforMax™ EPI300™-T1R Electrocompetent E. coli » Clone large inserts, control copy number of CopyControl™ vectors. Avoid T1 and T5 phage contamination.

BAC-Optimized Replicator™ v2.0 & 10G BAC-Optimized Electrocompetent Cells »Create BAC libraries.  Replicator cells control copy number of pSMART® BAC.

BigEasy®-TSA™ Electrocompetent Cells »Transform and propagate pJAZZ® Vector clones and libraries.

CJ236 Electrocompetent Cells » High efficiency cells to create uracil-containing DNA for site-directed mutagenesis.

Protein Expression

OverExpress™ C41(DE3) and C43(DE3) Competent Cells » Express difficult or even toxic proteins using any E. coli T7 vector. Available as chemically competent or electrocompetent cells.

E. cloni® EXPRESS BL21(DE3) Competent Cells » For routine protein expression using any E. coli T7 expression vector. Available as chemically competent or highest efficiency electrocompetent cells.

HI-Control™ BL21(DE3) and HI-Control™ 10G Competent Cells » Greater control of protein expression using the LacI repressor in two popular cell lines.