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TargetAmp™ Nano-g™ Biotin-aRNA Labeling Kit for the Illumina® System

TargetAmp™ Nano-g™ Biotin-aRNA Labeling Kit for the Illumina® System

货号

TAN091096

规格

96 Rxns

存储温度

-70℃

产品详情

由于生产线调整，自2017年1月1日起，此款产品转至Lucigen™品牌销售， （仅品牌变化，产品外包装变化，本身没有改变，老客户可以继续使用，实验不受影响）

 

Applications

  • Amplification and biotin-labeling of mRNA in total RNA of >2,500 cells (≥25 ng RNA) for use with Illumina® expression systems.

The TargetAmp™ Nano-g™ Biotin-aRNA Labeling Kit for the Illumina® System* produces biotin-aRNA (also called cRNA) for use with Illumina gene expression systems, such as the Illumina Expression BeadChip™ arrays (Fig. 1). TargetAmp Nano-g Kit reactions have been optimized to minimize sample size, and maximize biotin-aRNA yield and signal intensity. A TargetAmp Nano-g Kit reaction produces microgram amounts of biotin-labeled aRNA from as little as 25 ng of input total cellular RNA (Table 1) using an easy linear RNA amplification process. The biotin-aRNA produced by a TargetAmp Nano-g Kit reaction from 100 ng of input total RNA has been shown to detect more transcripts than biotin-aRNA produced by a competitive kit from 400 ng of input total RNA (Fig. 2).

Benefits

  • Easy 6-hour procedure yields purified biotin-aRNA ready for hybridization (Table 2).
  • Detects more genes using less input total RNA than competitive kits (Fig. 2).
  • Optimized for producing biotin-aRNA that enables high signal intensity on the Illumina BeadArray Reader.
  • Linear RNA amplification process preserves the relative abundance of the original sample.

Table 1. The TargetAmp™ Nano-g™ Kit produces microgram amounts of biotin-aRNA from as little as 25 ng of input total RNA. Results represent the average of multiple experiments.

Amount of Input HeLa Total RNA Biotin-aRNA Yield from HeLa Total RNAa
25 ng 3.1 µg
50 ng 5.3 µg
100 ng 10.8 µg
500 ng 75.6 µg

a. Approximately 2% of HeLa total RNA is poly(A) RNA.

Table 2. A TargetAmp™ Nano-g™ Kit procedure is faster and easier to perform than a competitive kit.

  TargetAmp™ Nano-g™ Kit Competitive Kit
First-Strand cDNA Synthesis 35 min 2 hr
Second-Strand cDNA Synthesis 13 min 2 hr
cDNA Purification Not necessary! 15-30 min
In Vitro Transcription 4.25 hr up to 14 hr
Biotin-aRNA Purification 15-30 min 15-30 min
Total Time Approximately 6 hr >Up to 36 hr
Figure 1
Figure 1. The TargetAmp™ Nano-g™ Kit uses a simplified, linear RNA amplification and labeling process that yields purified biotin-aRNA in about 6 hours.

Figure 2
Figure 2. Biotin-aRNA produced by a TargetAmp™ Nano-g™ Kit detects more genes using less input total RNA than biotin-aRNA produced by competitive kits. Biotin-aRNA produced from 100 ng of total RNA using the TargetAmp Nano-g kit and hybridized to the Illumina® MouseRef-8 Expression BeadChip™ detected 1,656 transcripts that were not detected using biotin-aRNA produced from 400 ng of total RNA using a competitive kit.

*TargetAmp™ aRNA Amplification Products are covered by intellectual property licensed to EPICENTRE Technologies Corporation from Johnson & Johnson Pharmaceutical Research & Development, L.L.C., and by intellectual property sublicensed to EPICENTRE Technologies Corporation from Incyte Corporation.

For research use only. Some applications in which this product may be used may be covered by patents or patent applications applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a license, depending upon the particular application and country in which the product is used.

 

Catalog No. Size
TargetAmp™ Nano-g™ Biotin-aRNA Labeling Kit for the Illumina® System
TAN07924 24 Reactions
TAN091096 96 Reactions
Contents: TargetAmp™ Nano-g™ T7-Oligo(dT) Primer, TargetAmp™ Nano-g™ 1st-Strand cDNA PreMix, TargetAmp™ Nano-g™ 2nd-Strand cDNA PreMix, TargetAmp™ Nano-g™ 2nd-Strand DNA Polymerase, TargetAmp™ Nano-g™ T7 RNA Polymerase, TargetAmp™ Nano-g™ T7 Transcription Buffer, TargetAmp™ Nano-g™ NTP PreMix, TargetAmp™ Nano-g™ UTP / Biotin-UTP, RNase-Free DNase I, DTT, HeLa Total RNA Control, RNase-Free Water.

 

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