MasterPure™ Yeast RNA Purification Kit

MasterPure™ Yeast RNA Purification Kit


货号

MPY03100


规格

100 Purifications


存储温度

Proteinase K and RNase-Free DNase I are stored at -20 C, the remainder is stored at +4℃


产品详情


 MasterPure™ Yeast RNA Purification Kit

MasterPure™ 酵母RNA纯化试剂盒

应用
  • 从酵母和丝状真菌纯化RNA用于cDNA合成和芯片表达谱分析(图1和2).
    MasterPure™ Yeast RNA Purification Kit 提供了从酵母中纯化RNA所需的所有试剂,可从假丝酵母,酿酒酵母,裂殖酵母和丝状真菌中提取。试剂盒使用快速的盐析过程去除污染大分子,不使用有机溶剂,玻璃珠和原生质体提取方法。
 
特点
  • 避免了热、酸、酚
  • 比原生质体提取法更快
  • 比玻璃珠提取法效果更好
  • 无需多余的酶或设备
  • 能够从1毫升中对数期的酿酒酵母中得到25-50 µg RNA

说明: http://www.epibio.com/images/default-source/product-page-images/g_masterpureyeast_purifiedyeast.jpg?sfvrsn=0.03597766093343191

说明: http://www.epibio.com/images/default-source/product-page-images/c_masterpureyeast_rnapurity.gif?sfvrsn=0.6470958098886063

Figure 1. MasterPure™ Yeast RNA Purification Kit提取RNA的芯片分析.RNA被反转录、标记并与酵母DNA芯片杂交。氧化应激下的细胞中的cDNACy5标记,对照细胞用Cy3标记。

Figure 2. MasterPure™ Yeast RNA Purification Kit提取的RNA纯度.1中的RNA -20° C 保存两个月后用Agilent Technologies 2100 Bioanalyzer®分析。电泳图显示了高纯度的、完整的RNA

 

产品编号     规格:
MasterPure™ Yeast RNA Purification Kit  酵母RNA纯化试剂盒
MPY03010 10 Reactions
MPY03100 100 Reactions
Contents: Extraction Reagent for RNA, MPC Protein Precipitation Reagent, TE Buffer (in 100-reaction kit only), Proteinase K, RNase-Free DNase I, RiboGuard™ RNase Inhibitor, 10X DNase Buffer, 2X T & C Lysis Solution.