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MasterAmp™ High Fidelity RT-PCR Kit

MasterAmp™ High Fidelity RT-PCR Kit

货号

RF91025

规格

25 Rxns

存储温度

-20℃

产品详情

 

Applications

  • Highly accurate RT-PCR amplification of RNA templates for cloning, sequencing, expression, and transcription analysis.
  • All-purpose RT-PCR in which full-length amplification and high accuracy are essential.
  • Multiplex RT-PCR.

The MasterAmp™ High Fidelity RT-PCR Kit is ideal for synthesis of accurate double-stranded cDNA products that will be used for cloning, sequencing, expression, or transcription analysis. The kit uses MMLV-RT Plus, a reverse transcriptase with an unsurpassed accuracy and reliability, and MasterAmp TAQurate™ DNA Polymerase, a high-fidelity PCR enzyme mix (Fig. 1). The kit also includes an optimized 2X RT-PCR Buffer PreMix (containing dNTPs, MgCl2, and buffer), random and oligo(dT) primers, and the MasterAmp PCR Enhancer with betaine,* which can substantially improve the yield and specificity of RT-PCR amplification.

Benefits

  • High-fidelity RT-PCR (i.e., low error rates).
  • Amplification of full-length products from RNA templates up to ~6 kb (Fig. 2).
  • Random and oligo(dT) primers included for first-strand cDNA synthesis.
  • Unique PreMix reduces pipetting steps and chances of contamination.
  • Uses MasterAmp PCR Enhancer for better amplification of high-GC or other difficult templates.

 

 

 

One-Step

Modified Two-Step

Standard Two-Step

Figure 1. Different RT-PCR protocols that can be used with the MasterAmp™ High Fidelity RT-PCR Kit.

 

 Figure 2. The MasterAmp™ High Fidelity RT-PCR Kit amplifies full-length products from RNA templates up to ~6 kb. RT-PCR products from Tobacco Mosaic Virus RNA with primers that amplify a 5.5-kb region of the viral RNA. Lane M, Kilobase ladder; lane 1, RT-PCR using the MasterAmp one-step protocol; lane 2, RT-PCR using MMLV-RT and Taq DNA polymerase with a standard two-step procedure; lane 3, RT-PCR using Tth DNA polymerase.

 

 

 Figure 3. Amplification of a variety of RNA templates using a one-step protocol with the MasterAmp™ High Fidelity RT-PCR Kit. Lane M, 100-bp ladder; lane 1, human lung β-actin; lane 2, human lung GAPDH; lane 3, region of TMV; lane 4, E. coli 16S rRNA; lane 5, human CGα; lane 6, human heart β-actin; lane 7, region of TMV. 

 


*Covered by issued and/or pending patents.

MasterAmp™ High Fidelity RT-PCR Kit. Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser’s own internal research. No other patent rights (such as 5´ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

 

Catalog No. Size
MasterAmp™ High Fidelity RT-PCR Kit
RF91025 25 Reactions
RF910100 100 Reactions
Contents: MMLV-RT Plus, MasterAmp™ TAQurate™ DNA Polymerase Mix, MasterAmp™ 2X RT-PCR PreMix (includes dNTPs), MasterAmp™ 10X RT-PCR Enhancer, Random Nonamer Primer, Oligo (dT)18 Primer, Control Template and Primer Mix, Sterile Water.

 

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