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ScriptSeq™ mRNA-Seq Library Preparation Kit (Roche Titanium-compatible)

ScriptSeq™ mRNA-Seq Library Preparation Kit (Roche Titanium-compatible)

货号

SST10096

规格

6 Rxns

存储温度

-20℃

产品详情

The ScriptSeq™ mRNA-Seq Library Preparation Kit (Roche Titanium-compatible)* uses a unique terminal-tagging technology* that simplifies strand-specific tagging and the preparation of directional mRNA-seq libraries from rRNA-depleted or poly(A)-enriched RNA (Fig. 1). The kit is suitable for any animal, plant, or bacterial species, and enables library preparation in about 3 hours.

The ScriptSeq™ mRNA-Seq Library Preparation Kit (Roche Titanium-compatible):

  • Enables strand-specific adaptor-tagging for directional sequencing using standard Roche 454 Titanium sequencing primers.
  • Produces emPCR-ready libraries in about 3 hours without the need for end-repair, adaptor ligations, shearing of the cDNA, or gel electrophoresis.
  • Uses random-primed cDNA synthesis to enable library preps from any animal, bacteria, or plant species.
  • Requires as little as 50 ng of rRNA-depleted RNA.

*Covered by issued and/or pending patents.

 

Figure 1

Table 1. The ScriptSeq™ mRNA-Seq Library Preparation Kit (Roche Titanium-compatible) generates libraries for directional sequencing in less than 3 hours. Times for each step are shown in hours:minutes.

Conventional mRNA-Seq Method ScriptSeq™ Method
Fragment RNA (1:00) Fragment RNA and synthesize di-tagged cDNA (1:40)*
Synthesize cDNA (4:30)
Ligate adaptors (2:00) Clean up cDNA (0:10)
Size-select from gel (1:30) —  
Enrich library by PCR (1:00) Enrich library by PCR (1:00)
Total time: 10:00 Total time:
*single-tube reaction		 ~3:00
Figure 1. Schematic overview of the ScriptSeq™ directional library preparation method. The process is complete in less than 3 hours, with no intermediate purification steps from RNA to di-tagged cDNA fragments.
A
Figure 2A		 B
Figure 2B		 Figure 2. Representative Bioanalyzer traces of input poly(A)+ and rRNA-depleted RNA, and corresponding ScriptSeq™ libraries. A) Poly(A)-enriched rat liver RNA; B) ScriptSeq library prepared from poly(A)-enriched rat liver RNA; C) rRNA-depleted Universal Human Reference RNA (UHRR) prepared using the Ribo-Zero™ Kit; D) ScriptSeq library prepared from Ribo-Zero rRNA-depleted UHRR.
C
Figure 2C		 D
Figure 2D


   Catalog No. Size
ScriptSeq™ mRNA-Seq Library Preparation Kit (Roche Titanium-compatible)
   SST10096 6 Reactions
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