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Nextera™ DNA Sample Prep Kit (Roche Titanium-compatible)
Nextera™ DNA Sample Prep Kit (Roche Titanium-compatible)
货号
NT09115
规格
5 Rxns
存储温度
-20℃
产品详情
Applications
- Preparation of DNA libraries compatible with Roche 454™ GS FLX and GS FLX Titanium™ next-generation sequencing instruments.
Nextera™ DNA Sample Prep Kits (Roche 454-Compatible) are specifically designed to prepare genomic DNA libraries compatible with Roche 454 GS FLX and GS FLX Titanium instruments. Nextera technology employs in vitro transposition* to simultaneously fragment and tag DNA in a single-tube reaction. The Nextera library preparation method offers significant advantages over existing methods, such as nebulization, sonication, or shearing (Table 1).
Benefits
- Only 50 ng of starting DNA required.
- Prepare sequencer-ready libraries in less than 2 hours.
- Incorporate platform-specific tags and optional bar codes (Fig. 1).
- Validated on Roche 454 GS FLX and GS FLX Titanium platforms.
*Covered by issued and/or pending patents.
Blue color indicates steps where the sample is transferred to another tube. |
Roche/454-Compatible
Figure 1 (click to enlarge). Generation of libraries for Roche 454™ platforms. After fragmenting and tagging genomic DNA with Nextera™ Enzyme Mix, four-primer limited-cycle PCR is performed which adds Roche 454-compatible adaptor sequences (blue and orange). Optional bar codes (triangle) can be added between the upstream emulsion PCR (emPCR) adaptor (blue) and the transposon end (gray). |
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For additional information on Nextera™ technology, please visit: www.epibio.com/nextera
For custom/bulk requests, please contact us at: Nextera@EpiBio.com
| Catalog No. | Size |
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|
|
| Nextera™ DNA Sample Prep Kit (Roche Titanium-Compatible) | |
| NT09115 | 5 Reactions |
| NT091120 | 20 Reactions |
| NT0911-50 | 50 Reactions |
| NT0911-96 | 96 Reactions |
|
Contents: Nextera Enzyme Mix (Roche Ti-Compatible), 5X Nextera Reaction Buffer (HMW), 50X Nextera Primer Cocktail (Roche Ti-Compatible), 50X Nextera Adaptor 1 (Roche Ti-Compatible), Nextera Control DNA, and 2X Nextera PCR Buffer. *Note: The Nextera™ PCR Enzyme (see below) must be purchased separately when using any of the Nextera™ DNA Sample Prep Kits. |
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| Nextera™ DNA Sample Prep Kit (Roche FLX-Compatible)* | |
| FL09115 | 5 Reactions |
| FL091120 | 20 Reactions |
|
Contents: Nextera Enzyme Mix (Roche FLX-Compatible), 5X Nextera Reaction Buffer (HMW), 50X Nextera Primer Cocktail (Roche FLX-Compatible), 50X Nextera Adaptor 1 (Roche FLX-Compatible), Nextera Control DNA, and 2X Nextera PCR Buffer. *Note: The Nextera™ PCR Enzyme (see below) must be purchased separately when using any of the Nextera™ DNA Sample Prep Kits. |
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| Nextera™ PCR Enzyme (required) | |
| EM091120 | 20 Reactions |
| EM091150 | 50 Reactions |
| EM0911-96 | 96 Reactions |
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| Nextera™ Bar Codes (optional) | |
| FLBC0950 | 12 Bar Codes (Roche FLX-Compatible) |
| NTBC0950 | 12 Bar Codes (Roche Titanium-Compatible) |
| Note: The bar coding kits are optional. Each kit contains 12 bar codes, and each bar code can be used to prepare up to 50 bar-coded libraries. | |
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