最新产品
Colony Fast-Screen™ Kit (PCR Screen)
Colony Fast-Screen™ Kit (PCR Screen)
货号
FS0322H
规格
200 Screens
存储温度
-20℃
产品详情
Applications
- Identification of desired clones in a library.
- Determining the orientation of cloned inserts.
- PCR of bacterial genomic DNA.
The Colony Fast-Screen™ Kit (PCR Screen) provides a rapid method for preparing clones for screening by PCR. Using the Colony Fast-Screen Kit (PCR Screen) there is no need to grow cultures or purify DNA prior to PCR (Fig. 1). The kit can be used with all standard
Benefits
- Identify desired clones by PCR without the need to grow cultures or purify DNA (Fig. 2).
- Enables PCR screening of high-copy clones, single-copy clones, and bacterial genomic DNA.
- Amenable to high-throughput applications.
- DNA preparation process does not interfere with pre-established PCR conditions.
- Enables both long and short PCR amplifications and can be used with all standard E. coli host strains.
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| Figure 1. Schematic overview of the protocol for the Colony Fast- Screen™ Kit (PCR Screen). | Figure 2. The Colony Fast-Screen™ Kit (PCR Screen) enables screening of both high-copy and single-copy clones by PCR without the need for cultures and DNA purifications. Colonies containing a 5-kb lambda fragment cloned into the CopyControl™ pCC1™ vector and grown on plates at either single-copy or high-copy number were picked and processed using the Colony Fast-Screen Kit (PCR Screen). PCR was performed using primers homologous to the ends of the cloning vector. Lane 1, DNA size marker; lane 2, E. coli DNA (negative control); lane 3, single-copy clone; lane 4, high-copy clone; lane 5, PCR of 1 ng of purified DNA. |
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