NTPhos™ Thermolabile Phosphatase

NTPhos™ Thermolabile Phosphatase




20,000 Units @ 20 U/μl






  • Removing 5´ phosphates from modified or unmodified NTPs, NDPs, NMPs, dNTPs, dNDPs, dNMPs, and inorganic pyrophosphate.
  • Removing ATP from enzymatic reactions.

NTPhos™ Thermolabile Phosphatase is a novel recombinant enzyme that is highly active in removing phosphate groups from the 5´ position of ribonucleoside- or 2´-deoxyribonucleoside- 5´-triphosphates, diphosphates, or monophosphates. It can be used to remove 5´-phosphate groups from nucleotide substrates following reactions using enzymes such as RNA-dependent or DNA-dependent RNA or DNA polymerases, terminal transferases, or poly(A) polymerases in order to prevent the nucleotides from inhibiting, interfering with, or causing background problems in subsequent downstream reactions.

Following removal of 5´ phosphates from nucleotides, the NTPhos enzyme can be completely and irreversibly heat-inactivated by incubation at 65°C for 15 minutes.

Unit Definition: One unit of NTPhos Thermolabile Phosphatase results in the release of 1 nmol of inorganic phosphate from ATP or dATP in 1 minute at 37°C under standard assay conditions.

Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM zinc acetate, 10 mM MgCl2, 0.1 M NaCl, and 0.1% Triton® X-100.

Quality Control: NTPhos Phosphatase is free of detectable DNA exo- and endonuclease and RNase activities.

Table 1. Nucleotides tested and hydrolyzed
by NTPhos™ Thermolabile Phosphatase
dATP ddATP 7-deaza-dATP 3´-dATP 2´-fluoro-dATP rATP rADP
dGTP ddGTP 7-deaza-dGTP     rGTP rGMP
dCTP ddCTP     2´-fluoro-dCTP rCTP rCDP
dTTP ddTTP          
dUTP       2´-fluoro-dUTP rUTP  
dNTP mix         rNTP mix  
Table 2. Nanomoles of inorganic phosphate released
from dATP upon NTPhos Phosphatase treatment at 37°C.
  Incubation time (in minutes) at 37°C
Units of NTPhos Phosphatase 5 min 10 min 15 min 20 min 25 min 30 min
1 Unit 67 116 153 178 196 213
2 Units 121 163 206 228 249 268
3 Units 128 199 217 246 264 273
4 Units 152 209 235 268 277 277
5 Units 169 222 259 270 291 297


Catalog No. Concentration Size

NTPhos™ Thermolabile Phosphatase
NT4910K 20 Units/µl 10,000 Units
NT4920K 20 Units/µl 20,000 Units