进口试剂

最新产品

NTPhos™ Thermolabile Phosphatase

NTPhos™ Thermolabile Phosphatase

货号

NT4920K

规格

20,000 Units @ 20 U/μl

存储温度

-20℃

产品详情

 

Applications

  • Removing 5´ phosphates from modified or unmodified NTPs, NDPs, NMPs, dNTPs, dNDPs, dNMPs, and inorganic pyrophosphate.
  • Removing ATP from enzymatic reactions.

NTPhos™ Thermolabile Phosphatase is a novel recombinant enzyme that is highly active in removing phosphate groups from the 5´ position of ribonucleoside- or 2´-deoxyribonucleoside- 5´-triphosphates, diphosphates, or monophosphates. It can be used to remove 5´-phosphate groups from nucleotide substrates following reactions using enzymes such as RNA-dependent or DNA-dependent RNA or DNA polymerases, terminal transferases, or poly(A) polymerases in order to prevent the nucleotides from inhibiting, interfering with, or causing background problems in subsequent downstream reactions.

Following removal of 5´ phosphates from nucleotides, the NTPhos enzyme can be completely and irreversibly heat-inactivated by incubation at 65°C for 15 minutes.

Unit Definition: One unit of NTPhos Thermolabile Phosphatase results in the release of 1 nmol of inorganic phosphate from ATP or dATP in 1 minute at 37°C under standard assay conditions.

Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM zinc acetate, 10 mM MgCl2, 0.1 M NaCl, and 0.1% Triton® X-100.

Quality Control: NTPhos Phosphatase is free of detectable DNA exo- and endonuclease and RNase activities.
Table 1. Nucleotides tested and hydrolyzed
by NTPhos™ Thermolabile Phosphatase
dATP ddATP 7-deaza-dATP 3´-dATP 2´-fluoro-dATP rATP rADP
dGTP ddGTP 7-deaza-dGTP     rGTP rGMP
dCTP ddCTP     2´-fluoro-dCTP rCTP rCDP
dTTP ddTTP          
dUTP       2´-fluoro-dUTP rUTP  
dNTP mix         rNTP mix  
Table 2. Nanomoles of inorganic phosphate released
from dATP upon NTPhos Phosphatase treatment at 37°C.
  Incubation time (in minutes) at 37°C
Units of NTPhos Phosphatase 5 min 10 min 15 min 20 min 25 min 30 min
1 Unit 67 116 153 178 196 213
2 Units 121 163 206 228 249 268
3 Units 128 199 217 246 264 273
4 Units 152 209 235 268 277 277
5 Units 169 222 259 270 291 297

 

Catalog No. Concentration Size
NTPhos™ Thermolabile Phosphatase
NT4910K 20 Units/µl 10,000 Units
NT4920K 20 Units/µl 20,000 Units
北京中北林格科技发展有限公司

 北京市昌平区北清路生命科学园;  Tel：010-88891086，80794780   Fax：010-80794780 
咨询订购QQ: 1443274331      邮件订购：bjzblg@126.com      
www.bjzblg.com   www.immunoreagents.cn  www.lucigen.com.cn
版权所有 @北京中北林格科技发展有限公司   京ICP备13002510号-1       网站建设：中企动力 北京   SEO标签