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EZ-Tn5™ <oriV /KAN-2> Insertion Kit
EZ-Tn5™ <oriV /KAN-2> Insertion Kit
货号
EZI02VK
规格
10 Rxns
存储温度
-20℃
产品详情
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Applications
The EZ-Tn5™ <oriV/KAN-2> Insertion Kit* randomly inserts a single EZ-Tn5™ <oriV/KAN-2> Transposon containing an inducible, high-copy origin of replication (oriV) into the target DNA. Thus, the kit integrates CopyControl™ capability into those vectors that lack it. TransforMax™ EPI300™ or EPI300™-T1R Electrocompetent E. coli are transformed with an aliquot of the reaction and selected for kanamycin resistance encoded by the EZ-Tn5 Transposon. Selected clones are amplified by adding the CopyControl Induction or Autoinduction Solution, and microgram quantities of template DNA can be purified from as little as 1 ml of culture. |
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Figure 1 (click to enlarge). The process for generating EZ-Tn5™ <oriV/KAN-2> Transposon insertion clones for high yields of DNA and bidirectional sequencing. |
| Catalog No. | Size |
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| EZ-Tn5™ <oriV/KAN-2> Insertion Kit | |
| EZI02VK | 10 Reactions |
| Contents: EZ-Tn5™ Transposase, EZ-Tn5™ <oriV/KAN-2> Transposon, EZ-Tn5™ 10X Reaction Buffer, EZ-Tn5™ 10X Stop Solution,Forward and Reverse Primers, Control Target DNA, Sterile Water. | |
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