MessageMAX™ T7 ARCA-Capped Message Transcription Kit

MessageMAX™ T7 ARCA-Capped Message Transcription Kit


货号

MMA60710


规格

10 Rxns


存储温度

-20℃


产品详情


The MessageMAX™ T7 ARCA-Capped Message Transcription Kit is optimized to produce the highest yield of RNA having an Anti-Reverse Cap Analog (ARCA) (m27,3’-OG[5']ppp[5']G) cap on the 5' end. A standard 30-minute, 20-μl Message-MAX reaction yields up to 60 μg of RNA starting with 1 μg of control template DNA. These yields are made possible by the unmatched properties of the MessageMAX T7 Enzyme Solution which allow
it to function in the presence of high concentrations of NTPs. Unlike ARCA, conventional m7G[5']ppp[5']G cap analog is asymmetrical and up to 50% of the capped RNA produced can have the cap in the reverseorientation which produces sub-optimal results in both in vitro and in vivo translation.1 Since the ARCA molecule included in the MessageMAX T7 ARCA-Capped Message Transcription Kit cannot be incorporated in the reverse orientation,100% of the caps in the RNA produced are in thecorrect orientation, increasing the translation efficiency of the RNA.2 The MessageMAX Kit contains a convenient ARCA Cap/NTP PreMix in which all four ribonucleotides and the cap analog are mixed in a single solution. The PreMix ensures the optimal concentration of each NTP and maintains the ideal ratio of ARCA to GTP (4:1) to drive the initiation of transcription with an ARCA cap while still producing high RNA yields.

Message Transcription Kit Contents
The MessageMAX™ Kit contains sufficient
reagents to perform 10 reactions.
MessageMAX™ T7 Enzyme
Solution................................................. 20 μl
ARCA Cap/NTP PreMix.......................... 80 μl
20 mM GTP ............................................ 20 μl
MessageMAX™ 10X
Transcription Buffer ................................ 20 μl
100 mM Dithiothreitol (DTT) ................... 20 μl
ScriptGuard™ RNase Inhibitor ............... 10 μl
RNase-Free Water ...................................1 ml
Control Template DNA
@ 0.5 μg/μl ............................................. 2 μg
RNase-Free DNase I .............................. 10 μl