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RepliPHI™ Phi29 Polymerase Dilution Buffer
RepliPHI™ Phi29 Polymerase Dilution Buffer
货号
RPB04041
规格
1 ml
存储温度
-20℃
产品详情
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RepliPHI™ Phi29 DNA Polymerase (φ29 DNA Polymerase) is a highly processive enzyme with exceptional strand-displacement activity. The enzyme also contains a 3´→5´ exonuclease activity that enables proofreading capability. Specific Activity: 1 x 106 Units/mg. Storage Buffer: RepliPHI™ Phi29 DNA Polymerase is supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.5% NP-40, and 0.5% Tween 20. Unit Definition: One unit of RepliPHI™ Phi29 DNA Polymerase converts 25 pmol of dNTPs into acid-insoluble material in 30 minutes at 30°C under standard assay conditions. Reaction Buffer: A Reaction Buffer is not supplied with RepliPHI Phi29 DNA Polymerase; however, a suggested Reaction Buffer is 40 mM Tris-HCl (pH 7.5), 50 mM KCl, 10 mM MgCl2, 5 mM (NH4)2SO4, and 4 mM DTT. A 10X Reaction Buffer is supplied with the RepliPHI Phi29 Reagent Sets. Quality Control: RepliPHI Phi29 DNA Polymerase is free of detectable endonuclease and RNase activities. RepliPHI Phi29 DNA Polymerase is free of detectable DNA contamination. |
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| Catalog No. | Concentration | Size |
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| RepliPHI™ Phi29 DNA Polymerase (enzyme only) | ||
| PP031010 | 1 µg/µl (1,000 U/µl) | 10 µg (10,000 Units) |
| PP040110 | 0.1 µg/µl (100 U/µl) | 10 µg (10,000 Units) |
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| RepliPHI™ Phi29 Reagent Set (includes enzyme, buffer, dNTPs, DTT) | ||
| RH031110 | Enzyme: 1 µg/µl (1,000 U/µl) | Enzyme: 10 µg (10,000 Units) |
| RH040210 | Enzyme: 0.1 µg/µl (100 U/µl) | Enzyme: 10 µg (10,000 Units) |
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| RepliPHI™ Phi29 Polymerase Dilution Buffer | ||
| RPB04041 | 1 ml | |
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