mRNA-ONLY™ Prokaryotic mRNA Isolation Kit

mRNA-ONLY™ Prokaryotic mRNA Isolation Kit




10 Purifications





  • Isolation of prokaryotic mRNA that is substantially free of contaminating rRNA for gene expression analysis, ribonuclease protection assays, RT-PCR, Northern blots, or other procedures requiring mRNA.
  • Production of prokaryotic cDNA libraries or isolation of specific cDNAs.

The mRNA-ONLY™ Prokaryotic mRNA Isolation Kit* provides a simple and effective method for isolating prokaryotic mRNA that is substantially free of ribosomal RNA (rRNA) in 1 hour. The mRNA-ONLY Kit includes EPICENTRE's Terminator™ 5´-Phosphate-Dependent Exonuclease,* a processive 5´→3´ exonuclease that digests RNA having a 5´ monophosphate. Bacterial rRNAs, which are transcribed as a single transcript and then processed to yield rRNAs with 5´ monophosphates are substrates for the enzyme. Thus, the mRNA-ONLY Prokaryotic mRNA Isolation Kit can be used to isolate prokaryotic mRNA substantially free of 16S and 23S rRNA.


  • The kit can be used to simultaneously prepare mRNA from mixed populations of prokaryotes or from mixed populations of both prokaryotic and eukaryotic cells, such as occur in biological samples involving symbiotic, parasitic, or pathogenic interactions (e.g., nitrogen fixation, prokaryotic infections, biological films, etc.).

*Covered by issued and/or pending patents.


Catalog No. Size

mRNA-ONLY™ Prokaryotic mRNA Isolation Kit
   MOP51010 10 Purifications
   MOP51024 24 Purifications
Contents: Terminator™ 5´-Phosphate-Dependent Exonuclease, mRNA-ONLY™ Prokaryotic 10X Reaction Buffers A and B, RiboGuard™ RNase Inhibitor, mRNA-ONLY™ Stop Solution, 5 M LiCl Solution, RNase-Free Water.