最新产品
mRNA-ONLY™ Eukaryotic mRNA Isolation Kit
mRNA-ONLY™ Eukaryotic mRNA Isolation Kit
货号
MOE51010
规格
10 Purifications
存储温度
-20℃
产品详情
Applications
- Quick and easy isolation of eukaryotic mRNA that is substantially free of contaminating rRNA, for gene expression analysis, ribonuclease protection assays, RT-PCR, Northern blots, or other procedures.
- Simplified generation of high-quality cDNA libraries.
Eukaryotic mRNA that is 3´-polyadenylated has traditionally been isolated by elution from immobilized oligo(dT) or oligo(dU) substrates. The mRNA-ONLY™ Eukaryotic mRNA Isolation Kit* provides a simple and effective method for isolating eukaryotic mRNA that is substantially free of ribosomal RNA (rRNA), in less than an hour, without binding to an oligo(dT) substrate.
The mRNA-ONLY Kit includes EPICENTRE's Terminator™* 5´-Phosphate-Dependent Exonuclease, a processive 5´→3´ exonuclease that digests RNA having a 5´ monophosphate. The enzyme does not digest RNA having a 5´ triphosphate, a 5´ cap (present on most eukaryotic mRNAs), or a 5´-hydroxyl group. These enzymatic properties make Terminator Exonuclease ideal for producing mRNA-enriched samples from eukaryotic total RNA preparations by selectively digesting the rRNAs (which have a 5´ monophosphate). Thus, the mRNA-ONLY Eukaryotic mRNA Isolation Kit can be used to isolate eukaryotic mRNA substantially free of 5.8S, 18S, and 28S rRNA. Transfer RNA (tRNA), and 5S rRNA are not removed by Terminator Exonuclease digestion.
Benefits
- No oligo(dT)-binding step is required, minimizing losses of mRNA due to inefficient binding.
- The mRNA-ONLY Eukaryotic mRNA Isolation Kit can be used to simultaneously prepare mRNA from both prokaryotic and eukaryotic cells.
*Covered by issued and/or pending patents.
| mRNA-ONLY™ Eukaryotic mRNA Isolation Kit | |
| MOE51010 | 10 Purifications |
| MOE51024 | 24 Purifications |
| Contents: Terminator™ 5´-Phosphate-Dependent Exonuclease, mRNA-ONLY™ Eukaryotic 10X Reaction Buffer A, RiboGuard™ RNase Inhibitor, mRNA-ONLY™ Stop Solution, 5 M LiCl Solution, RNase-Free Water. | |
北京市昌平区北清路生命科学园; Tel：010-88891086，80794780 Fax：010-80794780
咨询订购QQ: 1443274331 邮件订购：bjzblg@126.com
www.bjzblg.com www.immunoreagents.cn www.lucigen.com.cn
版权所有 @北京中北林格科技发展有限公司 京ICP备13002510号-1 网站建设：中企动力 北京 SEO标签