最新产品
BAC-Tracker™ Supercoiled DNA Ladder
BAC-Tracker™ Supercoiled DNA Ladder
货号
BT010950
规格
50 gel lanes
存储温度
-20℃ or +4℃ (See Literature)
产品详情
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Applications
The BAC-Tracker™ Supercoiled DNA Ladder is designed for estimating the size of large supercoiled DNAs, such as Bacterial Artificial Chromosome (BAC) clones, by agarose gel electrophoresis. The BAC-Tracker Ladder contains seven discrete supercoiled DNAs ranging from 8 kb to 165 kb (Fig. 1). The 8-kb band facilitates screening for "empty" or nonrecombinant BAC clones. The BAC-Tracker Supercoiled DNA Ladder is provided in a ready-to-load solution. Simply load 10 µl of BAC-Tracker Supercoiled DNA Ladder per gel lane. Run the gel, and stain using SYBR® Gold. The BAC-Tracker Ladder can be used with the Colony Fast-Screen™ Kit (Size Screen) to estimate the size of BAC clones in less than 4 hours. Quality Control: Each batch of BAC-Tracker Supercoiled DNA Ladder is checked by gel electrophoresis to ensure the correct size of all seven supercoiled DNA bands. |
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Figure 1. Supercoiled BAC DNA is sized by comparison with the BAC-Tracker™ Supercoiled DNA Ladder. Colonies from three different BAC clones (lanes 1-3) were processed using the Colony Fast-Screen™ Kit (Size Screen) and analyzed on a 1% agarose gel. Lane M, BAC-Tracker Supercoiled DNA Ladder. |
| Catalog No. | Size |
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| BAC-Tracker™ Supercoiled DNA Ladder | |
| BT010950 | 500 µl |
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