ScriptSeq™ Complete Kit (Human/Mouse/Rat)

ScriptSeq™ Complete Kit (Human/Mouse/Rat)


货号

BHMR1205


规格

6 Rxns


存储温度

-70℃


产品详情


ScriptSeq™ Complete Kits (人/大鼠/小鼠)

RNA测序文库制备试剂盒(人/大鼠/小鼠)

应用

  • 从少至100ng的总RNA制备测序文库,可用于Illumina公司®测序平台的使用。

The ScriptSeq™ Complete Kit 试剂盒结合了EPI公司Ribo-Zero™技术和ScriptSeq v2 RNA快速测序文库制备试剂盒的无缝末端连接技术,可在1天内实现RNA测序文库的制备(图1)。The ScriptSeq Complete Kits需要1-5微克的RNA,the ScriptSeq™ Complete Kits—Low Input 需要100ng-1ug的总RNA.
The ScriptSeq Complete Kit 包括 Ribo-Zero™试剂可去除细胞质(核编码)的rRNA,还包括零核糖金装试剂,可去除细胞质和线粒体核糖体RNA(图2)

特点:

  • 实现完整的单日RNA测序文库制备。
  • 产生方向性的RNA测序文库, 通常含<3%的rRNA读长序列。
  • 使用低至100 ng的总RNA,包括FFPE RNA。
  • 条码/标签兼容:ScriptSeq指数引物可单独编制有条形码库。

图1ScriptSeq™ Complete Kits过程概述
可在1天时间内完成从低至100 ng的总RNA,包括FFPE RNA,的高通量测序RNA文库制备,几乎不含有rRNA.

 

2 使用the ScriptSeq™ Complete Kit (Human/Mouse/Rat)—Low Input the ScriptSeq Complete Gold Kit (Human/Mouse/Rat)—Low Input. FFPE RNA 样品中制备的RNA测序文库评价。The ScriptSeq Complete Kit 包括 Ribo-Zero™试剂可去除细胞质(核编码)的rRNA,还包括零核糖金装试剂,可去除细胞质和线粒体核糖体RNA

 

 

货号     规格
ScriptSeq™ Complete Kit (Human/Mouse/Rat)
BHMR1205 6 Reactions
BHMR1224 24 Reactions
Note: For use with 1-5 µg total RNA.
包含:Ribo-Zero™ rRNA Removal Reagents (Human/Mouse Rat), Magnetic Core Kit, ScriptSeq™ v2 RNA-Seq Library Preparation Kit.三部分
ScriptSeq™ Complete Gold Kit (Human/Mouse/Rat)
BG1206 6 Reactions
BG1224 24 Reactions
Note: For use with 1-5 µg total RNA. Contents: One box of Ribo-Zero™ Gold rRNA Removal Reagents (Human/Mouse Rat), one box of Magnetic Core Kit, and one box of ScriptSeq™ v2 RNA-Seq Library Preparation Kit.
ScriptSeq™ Complete Kit (Human/Mouse/Rat)—Low Input
SCL24H 24 Reactions
SCL6H 6 Reactions
Note: For use with 100 ng to 1 µg total RNA. Contents: One box of Ribo-Zero™ rRNA Removal Reagents (Human/Mouse Rat)—Low Input, one box of Magnetic Core Kit—Low Input, and one box of ScriptSeq™ v2 RNA-Seq Library Preparation Kit.
ScriptSeq™ Complete Gold Kit (Human/Mouse/Rat)—Low Input
SCL24G 24 Reactions
SCL6G 6 Reactions
Note: For use with 100 ng to 1 µg total RNA. Contents: One box of Ribo-Zero™ Gold rRNA Removal Reagents (Human/Mouse/Rat)—Low Input, one box of Magnetic Core Kit—Low Input, and one box of ScriptSeq™ v2 RNA-Seq Library Preparation Kit.
FailSafe™ PCR Enzyme Mix
FSE51100 100 Units
The FailSafe™ PCR Enzyme Mix is required for library preparation.