进口试剂

最新产品

PlasmidMAX™ DNA Isolation Kit

PlasmidMAX™ DNA Isolation Kit

货号

PMX51050

规格

50 Preps

存储温度

-20℃

产品详情

Applications

  • Purification of plasmid clones for DNA sequencing or gene subcloning.

The PlasmidMAX™ DNA Isolation Kit purifies plasmids free from RNA and genomic DNA without the use of columns or hazardous chemicals, using a modified alkaline-lysis procedure. This simple procedure requires only two 1.5-ml tubes, thus avoiding excess plastic waste. The purification method is scaleable to larger volumes, so separate kits are not needed for varying sample sizes. For plasmids 3-40 kb, average sequence reads of >600 bases are obtained from DNA isolated using the PlasmidMAX Kit.

Benefits

  • Simple, rapid miniprep-type procedure.
  • High yields of highly purified plasmid DNA.
  • Scalable to larger volumes.
  • No toxic organic solvents.

 

Figure 1. High-quality sequence data is obtained from plasmid clones purified using the PlasmidMAX™ DNA Isolation Kit.

PlasmidMAX Kit Process

1. Pellet cells. Resuspend in Solution 1.

2. Add Solution 2. Mix.

3. Add Solution 3. Mix.

4. Pellet cell debris and chromosomal DNA.

5. Isopropanol precipitate nucleic acid.

6. Digest RNA with RiboShredder™ RNase.

7. Precipitate and wash plasmid DNA.

 

Figure 2. Agarose gel of plasmid DNAs purified using the PlasmidMAX™ Kit. Lanes 1-3, 4.5 kb plasmid clones; Lane 4, Supercoiled kb ladder.

 

Catalog No. Size
PlasmidMAX™ DNA Isolation Kit
PMX51050 50 Minipreps
Contents: PlasmidMAX™ Solutions 1-3, RiboShredder™ RNase Blend, RiboShredder™ Buffer, Plasmid Precipitation Solution, TE Buffer.

 

北京中北林格科技发展有限公司

 北京市昌平区北清路生命科学园;  Tel：010-88891086，80794780   Fax：010-80794780 
咨询订购QQ: 1443274331      邮件订购：bjzblg@126.com      
www.bjzblg.com   www.immunoreagents.cn  www.lucigen.com.cn
版权所有 @北京中北林格科技发展有限公司   京ICP备13002510号-1       网站建设：中企动力 北京   SEO标签