最新产品
AmpliCap™ T7 High Yield Message Maker Kit
AmpliCap™ T7 High Yield Message Maker Kit
货号
AC0707
规格
25 Rxns
存储温度
-20℃
产品详情
Applications
- Generate 5´-capped RNA from DNA cloned downstream of a phage T7 RNA Polymerase promoter for in vitro translation and microinjection.
- Functional studies of heterogenous nuclear RNAs and viral RNAs.
The AmpliCap™ T7 High Yield Message Maker Kit is optimized to produce high yields of RNA having a standard m7Gp3G cap on the 5´ end.
Benefits
- Yield up to 45 µg of RNA from 1 µg of T7 control template in a 20-µl reaction in 2 hours.
- Capping efficiencies up to 80%.
- An optimized m7G[5´]ppp[5´]G Cap/NTP PreMix is provided for ease of use and highest yields of capped RNA transcripts.
- A separate vial of GTP is provided for efficient production of long, 5´-capped RNA.
- Formulated to use high concentrations of NTPs that are inhibitory to conventional in vitro transcription kits.
- RNase inhibitor included.
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