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AmpliScribe™ T7 Aminoallyl-RNA Transcription Kit

AmpliScribe™ T7 Aminoallyl-RNA Transcription Kit

货号

AA50125

规格

25 Rxns

存储温度

-20℃

产品详情

Applications

  • Production of nonradioactive RNA probes for any purpose, including in situ hybridization and blotting experiments.

The AmpliScribe™ T7 Aminoallyl-RNA Transcription Kit enables high-yield synthesis of aminoallyl-labeled RNA (AA-RNA). The kit incorporates 5-(3-aminoallyl)-UTP (AA-UTP) into the RNA transcript. Since AA-UTP is incorporated into RNA with the same efficiency as UTP, high yields of AA-RNA are obtained. The AA-RNA product can then be treated with amine-reactive biotinylation reagents or fluorescent dyes to obtain biotin-labeled or fluorescently labeled RNA, respectively. Reaction conditions have been optimized to consistently generate uniformly labeled AA-RNA that, upon conjugation to a fluorescent dye or biotin compound, yields high signal intensity. This method for making nonradioactively labeled RNA is more efficient and less expensive than direct incorporation of fluorescent- or biotin-labeled NTPs into RNA during in vitro transcription.

 

Note: TargetAmp™ Aminoallyl-aRNA Amplification Kits are recommended for making labeled target for hybridizing to DNA microarrays.

Benefits

  • The kit uses EPICENTRE's AmpliScribe high-yield in vitro transcription technology to generate the highest possible yield of aminoallyl-RNA.
  • In vitro transcription reaction conditions are optimized to yield optimal levels of uniformly labeled AA-RNA for high signal intensity after dye or biotin conjugation.
  • More efficient and less expensive than direct incorporation of fluorescent- or biotin-labeled NTP.
  • RNase Inhibitor included.

Table 1. The AmpliScribe™ T7 Aminoallyl-RNA Transcription Kit produces very high yields of aminoallyl-RNA even from limiting amounts of DNA template.

 

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