最新产品
MessageMuter™ shRNA Production Kit
MessageMuter™ shRNA Production Kit
货号
MM031110
规格
10 Rxns
存储温度
-20℃
产品详情
The MessageMuter shRNA Production Process
The MessageMuter shRNA Production Kit utilizes a simple and unique 3-step process (Figure 2) that yields shRNA in about 4 hours. A 17-base T7 Promoter Oligo is annealed to a DNA oligo designed by the user using detailed instructions provided. The ends of the annealed duplex are "filled-in" using the Exo-Minus Klenow to obtain a linear dsDNA with a T7 RNAP promoter at its 5'-end. Following in vitro transcription, the RNA produced spontaneously forms a hairpin structure (shRNA). Following clean-up, the shRNA is ready for transfection into cultured cells. The MessageMuter shRNA kit provides reagents to produce 10 shRNAs in sufficient quantities for hundreds of transfections.
Applications
- Production of shRNA, micro RNA (miRNA) studies, or other applications.
Benefits
- Produces shRNA in vitro using one user-designed oligo and one in vitro transcription reaction.
- No need for annealing of sense and anti-sense RNA strands as required by other in vitro transcription-based methods.
 |
Figure 1. Short hairpin RNA (shRNA) directed against firefly luciferase. The shRNA produced using the MessageMuter™ shRNA Production Kit contains a sequence homologous to the target mRNA (sense sequence), a "loop" region and a sequence complementary to the target sequence (anti-sense sequence). |
 |
Figure 2. Overview of the method used to produce shRNA using the MessageMuter™ shRNA Production Kit. All reagents are supplied except a 60- to 76-base DNA oligo designed by the user as specified in the kit. |
- McManus, M.T. (2002) RNA 8 , 842.
- Paddison, P.J. et. al. (2002) Genes & Development 16, 948.
- Yu, J-Y et. al. (2002) Proc. Natl. Acad. Sci. USA 99 (9), 6047.
- Meis, J.E. (2003) EPICENTRE Forum 10 (2), 1.
- Meis, J.E. (2004) EPICENTRE Forum 11 (1), 1.
- Lin, Z.P. et. al. (2008) Mol. Pharmacol. 73, 243
Use of dsRNA for RNA interference by for-profit organizations requires a license from the Carnegie Institution of Washington. For-profit institutions should contact Gloria Brienza of the Carnegie Institution of Washington, 1530 P Street, N.W., Washington, D.C. 20005-1910. Email: gbrienza@pst.ciw.edu.
| Catalog No. | Size |
|
|
|
| MessageMuter™ shRNA Production Kit | |
| MM031110 | 10 Reactions |
|
Contents: T7 Promoter Oligo, Exo-Minus Klenow DNA Polymerase, 5X Annealing Buffer, dNTPs, AmpliScribe™ T7-Flash™ Enzyme Mix, AmpliScribe™ T7-Flash™ 10X Reaction Buffer, NTPs, DTT, RNase-Free Water, Control Oligo, and RiboGuard™ RNase Inhibitor. |
|
北京市昌平区北清路生命科学园; Tel：010-88891086，80794780 Fax：010-80794780
咨询订购QQ: 1443274331 邮件订购：bjzblg@126.com
www.bjzblg.com www.immunoreagents.cn www.lucigen.com.cn
版权所有 @北京中北林格科技发展有限公司 京ICP备13002510号-1 网站建设：中企动力 北京 SEO标签