Lambda Exonuclease is a highly processive 5´→3´ exodeoxyribonuclease that selectively digests the 5´ phosphorylated strand of dsDNA. The preferred substrate is blunt-ended, 5´-phosphorylated dsDNA. The enzyme has reduced activity against nicked DNA and against ssDNA and gapped DNA.3
Unit Definition: One unit of Lambda Exonuclease will produce 10 nmol of acid-soluble deoxyribonucleotides from dsDNA template in 30 minutes at 37°C in 1X Lambda Exonuclease Reaction Buffer.
Quality Control: Lambda Exonuclease is function-tested to ensure complete and preferential degradation of PCR products produced using 5´-phosphorylated primers. PCR products made using primers containing 5´-OH ends are not digested. Lambda Exonuclease is tested to be free of contaminating endonuclease activities.
|Figure. 1 Lambda Exonuclease selectively digests the strand of a PCR product produced using a PCR primer with a 5´-phosphate. The resulting single-stranded PCR product can be used for SSCP analysis or sequencing.|